Molecular and phenotypic characterization of near isogenic lines at QTL for quantitative resistance to Leptosphaeria maculans in oilseed rape (Brassica napus L.)

Autor: Cyril Falentin, N. Pouilly, R. Horvais, Nathalie Piel, P. Vallée, Claude Domin, Maurice Renard, Maria J. Manzanares-Dauleux, Régine Delourme
Přispěvatelé: Amélioration des Plantes et Biotechnologies Végétales (APBV), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Unité mixte de recherche interactions plantes-microorganismes, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2008
Předmět:
0106 biological sciences
Arabidopsis
rust resistance
Brassica
comparative genomics
MESH: Genetic Markers
01 natural sciences
phoma stem canker
MESH: Plant Diseases
Leptosphaeria maculans
MESH: Arabidopsis
Cultivar
field-resistance
COLZA
trait loci
0303 health sciences
genetic-markers acgm
biology
Chromosome Mapping
food and beverages
General Medicine
Phenotype
MESH: Immunity
Innate
Biotechnology
Genetic Markers
MESH: Brassica napus
Quantitative Trait Loci
Blackleg
arabidopsis-thaliana
MESH: Ascomycota
Quantitative trait locus
MESH: Phenotype
03 medical and health sciences
Ascomycota
Botany
Genetics
medicine
Gene
Plant Diseases
030304 developmental biology
Canker
[SDV.GEN]Life Sciences [q-bio]/Genetics
Brassica napus
barley
medicine.disease
biology.organism_classification
Immunity
Innate
MESH: Quantitative Trait Loci
[SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding
stripe rust
Genetic marker
MESH: Chromosome Mapping
Agronomy and Crop Science
head blight resistance
010606 plant biology & botany
Zdroj: TAG Theoretical and Applied Genetics
TAG Theoretical and Applied Genetics, 2008, 117, pp.1055-1067
HAL
TAG Theoretical and Applied Genetics, Springer Verlag, 2008, 117 (7), pp.1055-67. ⟨10.1007/s00122-008-0844-x⟩
TAG Theoretical and Applied Genetics, 2008, 117 (7), pp.1055-67. ⟨10.1007/s00122-008-0844-x⟩
TAG Theoretical and Applied Genetics, Springer Verlag, 2008, 117, pp.1055-1067
ISSN: 0040-5752
1432-2242
DOI: 10.1007/s00122-008-0844-x⟩
Popis: International audience; The most common and eVective way to control phoma stem canker (blackleg) caused by Leptosphaeria maculans in oilseed rape (Brassica napus) is by breeding resistant cultivars. SpeciWc resistance genes have been identiWed in B. napus and related species but in some B. napus cultivars resistance is polygenic [mediated by quantitative trait loci (QTL)], postulated to be race non-speciWc and durable. The genetic basis of quantitative resistance in the French winter oilseed rape ‘Darmor’, which was derived from ‘Jet Neuf’, was previously examined in two genetic backgrounds. Stable QTL involved in blackleg resistance across year and genetic backgrounds were identi- Wed. In this study, near isogenic lines (NILs) were produced in the susceptible background ‘Yudal’ for four of these QTL using marker-assisted selection. Various strategies were used to develop new molecular markers, which were mapped in these QTL regions. These were used to characterize the length and homozygosity of the ‘Darmor-bzh’ introgressed segment in the NILs. Individuals from each NIL were evaluated in blackleg disease Weld trials and assessed for their level of stem canker in comparison to the recurrent line ‘Yudal’. The eVect of QTL LmA2 was clearly validated and to a lesser extent, QTL LmA9 also showed an eVect on the disease level. This work provides valuable material that can be used to study the mode of action of genetic factors involved in L. maculans quantitative resistance.
Databáze: OpenAIRE
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