Bioconjugation of Rod-Shaped Fluorescent Nanocrystals for Efficient Targeted Cell Labeling
| Autor: | Claudia Tortiglione, Roberto Cingolani, Sasanka Deka, Alessandra Quarta, Andrea Ragusa, Teresa Pellegrino, Angela Tino |
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| Přispěvatelé: | Quarta, Alessandra, Ragusa, Andrea, Deka, Sasanka, Tortiglione, Claudia, Tino, Angela, Cingolani, Roberto, Pellegrino, Teresa |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Confocal
Selenium Compound Biotin HeLa Cell Fluorescence chemistry.chemical_compound Folic Acid Electrochemistry Humans General Materials Science Viability assay cardiovascular diseases Selenium Compounds Spectroscopy Electrophoresis Agar Gel Bioconjugation Microscopy Confocal biology Surfaces and Interfaces Condensed Matter Physics Protein subcellular localization prediction Molecular biology Immunohistochemistry Nanostructures chemistry Zinc Compounds Quantum dot biology.protein Biophysics cardiovascular system Zinc Compound Spectrophotometry Ultraviolet HeLa Cells Avidin Human |
| Zdroj: | Langmuir (Online) (2009). doi:10.1021/la901831y info:cnr-pdr/source/autori:Alessandra Quarta,?, Andrea Ragusa,+, Sasanka Deka,? Claudia Tortiglione,§ Angela Tino,§ Roberto Cingolani,? and Teresa Pellegrino*,+,?/titolo:Bioconjugation of Rod-Shaped Fluorescent Nanocrystals for Efficient Targeted Cell Labeling/doi:10.1021%2Fla901831y/rivista:Langmuir (Online)/anno:2009/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| DOI: | 10.1021/la901831y |
| Popis: | In the present work, we report a three-step approach for the functionalization of CdSe/CdS core/shell and CdSe/CdS/ZnS double-shell quantum rods (QRs) with either biotin or folic acid. We carried out an in vitro study on cultured cells and fixed tissue sections in which the biofunctionalized QRs were compared with the more traditional CdSe/ZnS quantum dots (QDs), which were also functionalized with either biotin or folic acid. The QR and the QD samples exhibited the same specificity toward the targeting cells. In addition, due to the enhanced photoluminescence of the QRs with respect to QDs, a lower amount of rods was required to image cells. In immuno-localization experiments on rat brain tissue sections, biotin-functionalized QRs have shown the typical protein localization patterns expected both for neuronal enolase NSE and actin, confirming the specificity of the interaction of QRs with avidin, and the feasibility of these materials as fluorescent probes in tissue staining. In this specific targeting study, we could assess via the MTT test, a cell viability assay, the lower toxicity of the CdSe/CdS/ZnS QRs with respect to CdSe/CdS QRs. |
| Databáze: | OpenAIRE |
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