Isolation of the transposable element hupfer from the entomopathogenic fungus Beauveria bassiana by insertion mutagenesis of the nitrate reductase structural gene
| Autor: | Thierry Langin, Pierre Capy, G. Riba, P. Maurer, A. Réjasse |
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| Rok vydání: | 1997 |
| Předmět: |
Transposable element
Sequence analysis Molecular Sequence Data Restriction Mapping Transposases Beauveria bassiana Sequence alignment Biology Nitrate Reductase Polymerase Chain Reaction Fungal Proteins Open Reading Frames Viral Proteins Nitrate Reductases Endopeptidases Genetics Amino Acid Sequence Cloning Molecular DNA Fungal Molecular Biology Transposase Repetitive Sequences Nucleic Acid Base Sequence Sequence Homology Amino Acid fungi Structural gene Fungal genetics Exons Sequence Analysis DNA biology.organism_classification Nucleotidyltransferases Molecular biology DNA-Binding Proteins Mutagenesis Insertional Entomopathogenic fungus DNA Transposable Elements Mitosporic Fungi DNA Probes Sequence Alignment |
| Zdroj: | Molecular and General Genetics MGG. 256:195-202 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/s004380050561 |
| Popis: | A transposable element has been isolated from the entomopathogenic fungus Beauveria bassiana by trapping it in the nitrate reductase structural gene, which has been cloned from this species. The element had inserted in the first exon of the nia gene and appeared to have duplicated the sequence TA at the site of insertion. It was 3336 bp long with 30-bp imperfect, inverted, terminal repeats. The element, called hupfer, contained an open reading frame encoding a 321-amino acid protein similar to the IS630- or mariner-Tcl-like transposases, and a residual sequence of about 2 kb which was not significantly similar to any published sequence. There are fewer than five copies of this transposable element present per genome in the fungus. |
| Databáze: | OpenAIRE |
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