Generation of Human Breg-Like Phenotype with Regulatory Function In Vitro with Bacteria-Derived Oligodeoxynucleotides

Autor: Verónica Astrid Pérez-Fernández, Jorge Gallego-Valle, Rafael Correa-Rocha, Marjorie Pion
Rok vydání: 2018
Předmět:
0301 basic medicine
medicine.medical_treatment
Regulatory B cells
CD40 Ligand
Interleukin-1beta
Programmed Cell Death 1 Receptor
Biology
Article
Catalysis
lcsh:Chemistry
Inorganic Chemistry
Cell therapy
03 medical and health sciences
Antigens
CD
Receptors
Transferrin
medicine
Macrophage
Humans
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
CD86
B-Lymphocytes
CD40
Bacteria
Organic Chemistry
Interleukin
Breg-like B cells
Granulocyte-Macrophage Colony-Stimulating Factor
General Medicine
Immunotherapy
suppressive function
Computer Science Applications
Cell biology
Interleukin-10
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Oligodeoxyribonucleotides
IL-10-producing B cells
biology.protein
B7-1 Antigen
Leukocytes
Mononuclear
B7-2 Antigen
CD80
Zdroj: Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid
Consejería de Sanidad de la Comunidad de Madrid
International Journal of Molecular Sciences
Volume 19
Issue 6
International Journal of Molecular Sciences, Vol 19, Iss 6, p 1737 (2018)
Popis: Regulatory B cells (Bregs) participate in auto-tolerance maintenance and immune homeostasis. Despite their impact on many diseases and due to the difficulty to define them, knowledge about their origin and their physiological inducers is still unclear. The incomplete understanding about the generation of Bregs and their limited numbers in periphery make it difficult to develop Breg-based therapy. Therefore, identifying factors that promote their development would allow their ex-vivo production in order to create new immunotherapy. This project aims to test the capacity of several cytokines (Interleukin 1-beta (IL-1&beta
), Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF), and Cluster of differentiation 40 ligand (CD40L)) and bacteria-derived oligodeoxynucleotides (CpG-ODN), alone or in combination, to generate B cells with regulatory phenotype and function. We have demonstrated that the Breg-associated phenotypes were heterogeneous between one and other stimulation conditions. However, the expression of other markers related to Bregs such as IL-10, CD80, CD86, CD71, Programmed cell death-1 (PD-1), and Programmed death-ligand 1 (PD-L1) was increased when cells were stimulated with CpG alone or in combination. Moreover, stimulated B cells presented a suppressive function on autologous activated peripheral blood mononuclear cells (PBMC) proliferation. Therefore, this work is the first step to demonstrate the feasibility to induce functional Breg-like cells in vitro and will then facilitate the way to produce Breg-like cells as a potential future cellular therapy.
Databáze: OpenAIRE
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