Identification of the optimal method for removing the capsule from the acinus of the rat's mandibular glands when preparing specimens for superficial morphology examination
| Autor: | Rieko Kominami, Satoru Honma, Takamitsu Arakawa, Akimichi Takemura, Mamoru Uemura |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Morphology (linguistics) Scanning electron microscope Capsule Hydrochloric acid Submandibular gland Salivary Glands Specimen Handling 03 medical and health sciences chemistry.chemical_compound medicine.anatomical_structure stomatognathic system chemistry Acinus Collagenase medicine Animals 030101 anatomy & morphology Anatomy Rats Wistar medicine.drug Biomedical engineering |
| Zdroj: | Okajimas folia anatomica Japonica. 94(3) |
| ISSN: | 1881-1736 |
| Popis: | The superficial morphology of the acinus of the mandibular gland in rats, which corresponds to the submandibular gland in humans, is very difficult to observe under scanning electron microscope due to a closely adherent capsule. Therefore, we evaluated the most effective protocol for removing this capsule from the acinus using various solutions, at different temperatures and for different durations of soaking. Based on the data for 50 male Wistar rats, the most effective method was soaking in an 8 N hydrochloric acid solution at 60°C for 70 min, in a water bath, followed by soaking in a 0.1-0.2% collagenase solution at 37°C for 330-350 min. |
| Databáze: | OpenAIRE |
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