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The total area of Soybean Cyst Nematode (SCN, Heterodera glycines Ichinohe)-infected soybean (Glycine max (L.) Merr.) in China increases each year, resulting in substantial yield losses. It becomes more and more important to discovery and incorporate new gene into breeding program for developing resistant cultivars. In this paper, we introduced more than 400 resistant accessions which were resistant to race 1, 2, 3, 4 and 5, especially some ones with resistance to multiple races. These resistant accessions were used to construct SCN applied core collection that consisted of 29 accessions but could represent more than 70% of genetic diversity of the whole resistant collection. By using linkage and association mapping methods, 15 QTLs, 22 SSR markers, 5 SNP markers and 1 SNP haplotype were identified associated with resistance to SCN. Moreover, one resistant candidate gene GmHs1pro-1 was cloned and analyzed for sequence variation. In addition, 11 SNP markers were developed from candidate genes rhg1 and Rhg4 respectively, which had been used to analysis genetic diversity and genetic relationship among soybean accessions. However, it is essential to discover novel gene from the resistant accession and explore new breeding methods for accelerating SCN resistant soybean improvement. |
