Mageb4 , a Testis-Specific Gene, is Dispensable for Mouse Spermatogenesis
| Autor: | Xiaolong Wu, Yunhao Wu, Damin Yun, Dengfeng Lin, Sheng Gao, Fei Sun, Liwei Zhou |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
endocrine system lcsh:RC648-665 Obstetrics and Gynecology Biology medicine.disease lcsh:Diseases of the endocrine glands. Clinical endocrinology Male infertility clustered regularly interspaced palindromic repeats/crispr-associated protein 9 mageb4 male infertility spermatogenesis Andrology medicine.anatomical_structure Reproductive Medicine Knockout mouse Homologous chromosome medicine lcsh:RC581-607 Gene Spermatogenesis Germ cell Sperm motility Immunostaining |
| Zdroj: | Reproductive and Developmental Medicine, Vol 4, Iss 3, Pp 129-136 (2020) |
| ISSN: | 2589-8728 |
| DOI: | 10.4103/2096-2924.296546 |
| Popis: | Objective: It has recently been shown that the melanoma antigen gene (MAGE) family is expressed in various tumor cell lines but silent in normal tissues, except germ cell lines. Mageb4, a member of the MAGE family, is highly expressed in the testis and homologous in humans and mice. Whole-exome sequencing studies have identified Mageb4 as a possible X-linked cause of inherited male infertility. However, the function of Mageb4 protein remains largely unknown. Methods: Using clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) 9 technology, we generated a Mageb4 knockout mouse model (Mageb4−/Y) to explore the role of this gene in spermatogenesis. Results: First, immunostaining of testicular cells showed that Mageb4 is localized in the cytoplasm of spermatogonia. Second, Mageb4−/Y male mice displayed significant increases in apoptosis. However, Mageb4−/Y male mice showed normal fertility, including normal sperm concentration, sperm motility, and testicular and epididymal histology. Conclusions: These findings suggest that, despite testis-exclusive expression, Mageb4 is dispensable for mouse spermatogenesis. Future research should focus on the role of this gene in apoptosis, aiming to provide clinical guidance regarding male infertility. |
| Databáze: | OpenAIRE |
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