5-Fluorouracil Encapsulated Chitosan-Cellulose Fiber Bionanocomposites: Synthesis, Characterization and In Vitro Analysis towards Colorectal Cancer Cells
| Autor: | Hui-Yin Chan, Nor Azwadi Che Sidik, Sin-Yeang Teow, Mostafa Yusefi, Kamyar Shameli, Michiele Lee-Kiun Soon, Pooneh Kia |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biocompatibility
in vitro drug release Scanning electron microscope General Chemical Engineering Sodium chitosan nanoparticles bionanocomposites chemistry.chemical_element colorectal cancer cytotoxicity assay Article cellulose Chitosan Chemistry Cellulose fiber chemistry.chemical_compound chemistry Cancer cell General Materials Science Thermal stability 5-fluorouracil Cellulose QD1-999 Nuclear chemistry |
| Zdroj: | Nanomaterials Volume 11 Issue 7 Nanomaterials, Vol 11, Iss 1691, p 1691 (2021) |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano11071691 |
| Popis: | Cellulose and chitosan with remarkable biocompatibility and sophisticated physiochemical characteristics can be a new dawn to the advanced drug nano-carriers in cancer treatment. This study aims to synthesize layer-by-layer bionanocomposites from chitosan and rice straw cellulose encapsulated 5-Fluorouracil (CS-CF/5FU BNCs) using the ionic gelation method and the sodium tripolyphosphate (TPP) cross-linker. Data from X-ray and Fourier-transform infrared spectroscopy showed successful preparation of CS-CF/5FU BNCs. Based on images of scanning electron microscopy, 48.73 ± 1.52 nm was estimated for an average size of the bionanocomposites as spherical chitosan nanoparticles mostly coated rod-shaped cellulose reinforcement. 5-Fluorouracil indicated an increase in thermal stability after its encapsulation in the bionanocomposites. The drug encapsulation efficiency was found to be 86 ± 2.75%. CS-CF/5FU BNCs triggered higher drug release in a media simulating the colorectal fluid with pH 7.4 (76.82 ± 1.29%) than the gastric fluid with pH 1.2 (42.37 ± 0.43%). In in vitro cytotoxicity assays, cellulose fibers, chitosan nanoparticles and the bionanocomposites indicated biocompatibility towards CCD112 normal cells. Most promisingly, CS-CF/5FU BNCs at 250 µg/mL concentration eliminated 56.42 ± 0.41% of HCT116 cancer cells and only 8.16 ± 2.11% of CCD112 normal cells. Therefore, this study demonstrates that CS-CF/5FU BNCs can be considered as an eco-friendly and innovative nanodrug candidate for potential colorectal cancer treatment. |
| Databáze: | OpenAIRE |
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