Multiple gating modes and the effect of modulating factors on the microI sodium channel
| Autor: | William S. Agnew, Fred J. Sigworth, Jiuying Zhou, James S. Trimmer, Jerald F. Potts |
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| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Time Factors Xenopus Gating Biology Sodium Channels Injections Internal medicine medicine Animals Patch clamp Hyperkalemic periodic paralysis RNA Messenger Membrane potential General Neuroscience Sodium channel Muscles Skeletal muscle Brain Depolarization DNA medicine.disease Electrophysiology medicine.anatomical_structure Endocrinology Biophysics Oocytes Poly A Ion Channel Gating |
| Zdroj: | Neuron. 7(5) |
| ISSN: | 0896-6273 |
| Popis: | Macroscopic current from the μI skeletal muscle sodium channel expressed in Xenopus oocytes shows inactivation with two exponential components. The major, slower component's amplitude decreases with rapid pulsing. When μI cRNA is coinjected with rat skeletal muscle or brain mRNA the faster component becomes predominant. Individual μI channels switch between two principal gating modes, opening either only once per depolarization, or repeatedly in long bursts. These two modes differ in both activation and inactivation kinetics. There is also evidence for additional gating modes. It appears that the equilibrium among gating modes is influenced by a modulating factor encoded in rat rat skeletal muscle and brain mRNA. The modal gating is similar to that observed in hyperkalemic periodic paralysis. |
| Databáze: | OpenAIRE |
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