Preliminary cross-genera transferability of SSRs among threatened South American Cupressaceae
| Autor: | Andrea C. Premoli, Cintia Paola Souto, Mariana Fasanella |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Cupressaceae biology Transferability Pilgerodendron AUSTROCEDRUS MICROSATELLITES Plant Science FITZROYA biology.organism_classification 010603 evolutionary biology 01 natural sciences Ciencias Biológicas Geography Evolutionary biology South american Threatened species Botany Microsatellite PILGERODENDRON Austrocedrus Ecology Evolution Behavior and Systematics CIENCIAS NATURALES Y EXACTAS 010606 plant biology & botany Conservación de la Biodiversidad |
| Popis: | Development of specific microsatellite primers is a relatively expensive and time-consuming process, so transferring of already developed markers from one species to others is desirable, especially for non-model organisms with large genome structure such as conifers. In austral South America the three Cupressaceae are monotypic genera of high conservation and evolutionary relevance. Although, they had beenthe focus of numerous population genetic studies the lack of highly polymorphic markers as microsatellites for all species, has limited the possibility of evolutionary studies and the design of appropriate conservation plans. Thus the aim of this study is to transfer and test microsatellites primers early designed for Austrocedrus to Fitzroya and Pilgerodendron. Transferability of nine microsatellite primers and their utility were tested by comparing genetic parameters of 20 individuals of each species in six populations where the three species occur. Four markers were simultaneously transferred with success, representing the first study in conifers to achieve cross-genera transferability. This small set of primers yielded high levels of polymorphism for the three studied species and show unique alleles that separate pure taxa. These highly polymorphic markers provide relevant tools to study the evolutionary history of South American Cupressaceae and to generate information that could help to guide conservation efforts. Fil: Fasanella, Mariana. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Laboratorio de Ecotono; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina Fil: Souto, Cintia Paola. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Laboratorio de Ecotono; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina Fil: Premoli Il'grande, Andrea Cecilia. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Laboratorio de Ecotono; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina |
| Databáze: | OpenAIRE |
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