Cross-Platform DNA Encoding for Single-Cell Imaging of Gene Expression
| Autor: | Peter T. Nelson, Xiaohu Gao, David Wu, Jonathan R. Fromm, Konstantin Queitsch, Shreeram Akilesh, Lawrence D. True, Wanyi Tai, Pavel Zrazhevskiy, John A. Stamatoyannopoulos |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell physiology Cell Gene Expression Computational biology Catalysis Article 03 medical and health sciences chemistry.chemical_compound Single-cell analysis RNA interference Gene expression Quantum Dots medicine Humans RNA Messenger Fluorescent Dyes Messenger RNA Chemistry General Chemistry Transfection DNA General Medicine Molecular biology 030104 developmental biology medicine.anatomical_structure Microscopy Fluorescence Single-Cell Analysis HeLa Cells |
| Zdroj: | Angewandte Chemie. 128:9121-9124 |
| ISSN: | 0044-8249 |
| DOI: | 10.1002/ange.201603945 |
| Popis: | Integration of imaging data across different molecular target types can provide in-depth insight into cell physiology and pathology, but remains challenging owing to poor compatibility between target-type-specific labeling methods. We show that cross-platform imaging analysis can be readily achieved through DNA encoding of molecular targets, which translates the molecular identity of various target types into a uniform in situ array of ssDNA tags for subsequent labeling with complementary imaging probes. The concept was demonstrated through multiplexed imaging of mRNAs and their corresponding proteins with multicolor quantum dots. The results reveal heterogeneity of cell transfection with siRNA and outline disparity in RNA interference (RNAi) kinetics at the level of both the mRNA and the encoded protein. |
| Databáze: | OpenAIRE |
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