Berberine and Tinospora cordifolia exert a potential anticancer effect on colon cancer cells by acting on specific pathways
| Autor: | Camilla Baserga, Luca Scapoli, Manuela Mandrone, Ferruccio Poli, Annalisa Palmieri, Laura Mercolini, Marcella Martinelli, Aldo Bruno Giannì, Anastasia Iapichino |
|---|---|
| Přispěvatelé: | Palmieri, Annalisa, Scapoli, Luca, Iapichino, Anastasia, Mercolini, Laura, Mandrone, Manuela, Poli, Ferruccio, Giannì, Aldo Bruno, Baserga, Camilla, Martinelli, Marcella |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Colorectal cancer Metabolite Immunology colorectal cancer Tinospora cordifolia Pharmacology liquid chromatography–mass spectrometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Berberine Downregulation and upregulation berberine Gene expression medicine Immunology and Allergy biology Chemistry biology.organism_classification medicine.disease 030104 developmental biology Cell culture 030220 oncology & carcinogenesis gene expression Adenocarcinoma |
| Zdroj: | International Journal of Immunopathology and Pharmacology. 33:205873841985556 |
| ISSN: | 2058-7384 |
| DOI: | 10.1177/2058738419855567 |
| Popis: | Berberine (BBR) is a natural active principle with potential antitumor activity. The compound targets multiple cell signaling pathways, including proliferation, differentiation, and epithelial–mesenchymal transition. The aim of this study was to elucidate the mechanisms behind the anticancer activity of BBR by comparing the effects of purified BBR with those of the extract of Tinospora cordifolia, a medicinal plant that produces this metabolite. The expression levels of a panel of 44 selected genes in human colon adenocarcinoma (HCA-7) cell line were quantified by real-time polymerase chain reaction (PCR). BBR treatment resulted in a time- and dose-dependent down regulation of 33 genes differently involved in cell cycle, differentiation, and epithelial–mesenchymal transition. The trend was confirmed across the two types of treatment, the two time points, and the different absolute dosage of BBR. These findings suggest that the presence of BBR in T. cordifolia extract significantly contributes to its antiproliferative activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|