Interstitial Cells of Cajal Modulate the Tone of the Human Internal Anal Sphincter In Vitro
| Autor: | Alison F. Brading, Neil Mortensen, Bruno Lorenzi |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male medicine.medical_specialty Carbachol medicine.drug_class Urology Anal Canal Proto-Oncogene Mas Gastroenterology Piperazines Tyrosine-kinase inhibitor Internal anal sphincter Immunoenzyme Techniques symbols.namesake Internal medicine medicine Humans Protein Kinase Inhibitors Phenylephrine Aged Aged 80 and over business.industry General Medicine Middle Aged Interstitial Cells of Cajal Electric Stimulation Interstitial cell of Cajal Proto-Oncogene Proteins c-kit Pyrimidines Imatinib mesylate medicine.anatomical_structure Benzamides Imatinib Mesylate symbols Immunohistochemistry Sphincter Female business Muscle Contraction medicine.drug |
| Zdroj: | Diseases of the Colon & Rectum. 57:370-377 |
| ISSN: | 0012-3706 |
| DOI: | 10.1097/01.dcr.0000442896.47732.e8 |
| Popis: | Background Interstitial cells of Cajal, expressing the proto-oncogene c-kit, have been shown to regulate the spontaneous activity of the gastrointestinal tract. They have been described in the human internal anal sphincter; however, their function is still unclear. Objective We examined the effects of the c-kit tyrosine kinase inhibitor imatinib mesylate on sphincter strips to investigate the function of the interstitial cells. Design This was a case series study. Settigs This was a single-center study conducted at the University of Oxford. Patients Internal anal sphincter strips were collected from 10 patients undergoing abdominoperineal resection or proctectomy and mounted in organ bath. Responses to electrical field stimulation and chemical agents were monitored in the absence of drugs and after the administration of increasing doses of imatinib mesylate. Immunohistochemistry was performed to identify interstitial cells. Main outcome measures The role of the interstitial cells in the internal anal sphincter was assessed. Results Imatinib mesylate significantly reduced the tone and the spontaneous activity of the strips. In the absence of drugs, the tone generated was 147.7 ± 33.0 mg/mg of tissue. Administration of ≥5 μM of imatinib mesylate caused a dose-dependent reduction in the tone. Strips exhibited spontaneous activity characterized by intermittent low-amplitude contractions superimposed on basal tone (135.6 ± 4.6 contractions in 10 minutes). Imatinib mesylate significantly reduced the number of contractions at concentration >5 μM. No differences were observed in the responses to electrical field stimulation, carbachol, or phenylephrine. Immunohistochemistry showed c-kit-positive cells. Limitations This study was limited by the relatively small number of patients enrolled and thus the difficulty of finding human tissue for laboratory studies. Conclusions Our results suggest that the interstitial cells modulate the tone and the spontaneous activity of the internal anal sphincter. This provides a foundation for new approaches to preclinical and clinical research. Moreover, these cells may represent a target for drugs inhibiting the c-kit receptor and provide a new approach for treating anorectal diseases. |
| Databáze: | OpenAIRE |
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