Comparison of immune response in sheep immunized with DNA vaccine encoding Toxoplasma gondii GRA7 antigen in different adjuvant formulations
| Autor: | Józef Kur, Janet Boyu Xu, Hong Li, Roy Bickerstaffe, M. Stankiewicz, Gabriela Olędzka, Elżbieta Hiszczyńska-Sawicka |
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| Rok vydání: | 2009 |
| Předmět: |
animal diseases
medicine.medical_treatment Immunology Protozoan Proteins Antibodies Protozoan chemical and pharmacologic phenomena Antigens Protozoan CHO Cells Injections Intramuscular Microbiology DNA vaccination Interferon-gamma Random Allocation Plasmid Immune system Cricetulus Antigen Adjuvants Immunologic Cricetinae medicine Vaccines DNA Animals Sheep biology Toxoplasma gondii General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Virology Vaccination Infectious Diseases Immunoglobulin G Liposomes Models Animal biology.protein bacteria Parasitology Female Antibody Adjuvant Toxoplasma Plasmids |
| Zdroj: | Experimental parasitology. 124(4) |
| ISSN: | 1090-2449 |
| Popis: | Immunization with plasmid DNA, a relatively novel technique, is a promising vaccination technique. To improve the immune response by DNA vaccination various methods have been used, such as chemical adjuvants or immunomodulatory molecules formulated into microparticles or liposomes. The aim of this research is to evaluate the immune responses of sheep immunized with DNA plasmids encoding Toxoplasma gondii dense granule antigen GRA7 formulated into three different adjuvant formulations. Sixty sheep were injected intramuscularly with the DNA plasmids. Twelve received the liposome-formulated plasmid pVAXIgGRA7, 12 Emulsigen P formulated plasmid pVAXIgGRA7 and 12 Emulsigen D formulated plasmid pVAXIgGRA7. Twelve animals were used as a control and received the vector alone. All the animals were inoculated at week 0, and week 4. Immunization of the sheep with plasmids encoding GRA7, with the different adjuvant formulations, effectively primed the immune response. After the first inoculation, moderate to high antibody responses were observed with the three different adjuvant formulations. A significantly elevated specific IgG2 response was observed in the sheep immunized with liposomes and Emulsigen D as adjuvants. In the group immunized with Emulsigen P as an adjuvant, lower IgG1 and IgG2 antibody levels were developed compared to the other treatment groups. In all the immunized groups, DNA immunization stimulated a IFN-gamma response. No antibody or IFN-gamma responses were detected in the control group immunized with an empty plasmid or not immunized. These results indicate that intramuscular immunization of sheep with a DNA vaccine with the adjuvants liposomes and Emulsigen D induce a significant immune response against T. gondii. |
| Databáze: | OpenAIRE |
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