The Diaphragms of Fenestrated Endothelia: Gatekeepers of Vascular Permeability and Blood Composition
| Autor: | Randolph J. Noelle, Samantha Shipman, Marcus R. Luciano, Krishnamurthy V. Nemani, Raul Elgueta, Catherine Carrière, Karen L Moodie, Steven Fiering, Dan Tse, Nicole C. Smits, Barjor Gimi, Charles P. Daghlian, Hong-Kee Lee, Takashi Kobayashi, Sophie J. Deharvengt, Daniel S. Longnecker, Patricia Ernst, Radu V. Stan, Maarten Buitendijk, Caitlin L. McGarry, Nicholas W. Shworak, Alan R. Schned, Arief A. Suriawinata, Yan Xu |
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| Rok vydání: | 2012 |
| Předmět: |
Endothelium
Transgene Protein-Losing Enteropathies Vascular permeability Mice Transgenic Biology Caveolae General Biochemistry Genetics and Molecular Biology Article Cell membrane Capillary Permeability 03 medical and health sciences Mice 0302 clinical medicine medicine Animals Molecular Biology Barrier function 030304 developmental biology 0303 health sciences Cell Membrane Membrane Proteins Cell Biology Blood Proteins Diaphragm (structural system) Cell biology Capillaries medicine.anatomical_structure Membrane protein 030220 oncology & carcinogenesis Immunology Endothelium Vascular Carrier Proteins Developmental Biology |
| Zdroj: | Stan, R V, Tse, D, Deharvengt, S J, Smits, N C, Xu, Y, Luciano, M R, McGarry, C L, Buitendijk, M, Nemani, K V, Elgueta, R, Kobayashi, T, Shipman, S L, Moodie, K L, Daghlian, C P, Ernst, P A, Lee, H-K, Suriawinata, A A, Schned, A R, Longnecker, D S, Fiering, S N, Noelle, R J, Gimi, B, Shworak, N W & Carrière, C 2012, ' The diaphragms of fenestrated endothelia : gatekeepers of vascular permeability and blood composition ', Developmental Cell, vol. 23, no. 6, pp. 1203-18 . https://doi.org/10.1016/j.devcel.2012.11.003 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2012.11.003 |
| Popis: | Fenestral and stomatal diaphragms are endothelial subcellular structures of unknown function that form on organelles implicated in vascular permeability: fenestrae, transendothelial channels, and caveolae. PV1 protein is required for diaphragm formation in vitro. Here, we report that deletion of the PV1-encoding Plvap gene in mice results in the absence of diaphragms and decreased survival. Loss of diaphragms did not affect the fenestrae and transendothelial channels formation but disrupted the barrier function of fenestrated capillaries, causing a major leak of plasma proteins. This disruption results in early death of animals due to severe noninflammatory protein-losing enteropathy. Deletion of PV1 in endothelium, but not in the hematopoietic compartment, recapitulates the phenotype of global PV1 deletion, whereas endothelial reconstitution of PV1 rescues the phenotype. Taken together, these data provide genetic evidence for the critical role of the diaphragms in fenestrated capillaries in the maintenance of blood composition. |
| Databáze: | OpenAIRE |
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