Prokineticin-1 (Prok-1) works coordinately with glial cell line-derived neurotrophic factor (GDNF) to mediate proliferation and differentiation of enteric neural crest cells

Autor: Hiu-Ching Poon, Mai Har Sham, Cathy K.Y. Shum, Vincent C.H. Lui, Paul K.H. Tam, Elly Sau-Wai Ngan, Maria-Mercè Garcia-Barceló
Rok vydání: 2008
Předmět:
medicine.medical_specialty
Mice
Transgenic
Biology
Models
Biological
Enteric Nervous System
Receptors
G-Protein-Coupled
Mice
Neurotrophic factors
Internal medicine
medicine
Glial cell line-derived neurotrophic factor
Animals
Glial Cell Line-Derived Neurotrophic Factor
Molecular Biology
Neural crest cell
Cell Proliferation
Glycoproteins
Oligonucleotide Array Sequence Analysis
Extracellular Matrix Proteins
urogenital system
Microarray analysis techniques
Gene Expression Profiling
Proto-Oncogene Proteins c-ret
Gene Expression Regulation
Developmental
Neural crest
Cell Differentiation
Cell Biology
Prokineticin receptor 1
Embryo
Mammalian
GDNF
Prokineticin
Cell biology
Endocrinology
nervous system
Neural Crest
biology.protein
Vascular Endothelial Growth Factor
Endocrine-Gland-Derived
Enteric nervous system
GDNF family of ligands
Prok-1
Vasoactive Intestinal Peptide
Zdroj: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1783:467-478
ISSN: 0167-4889
DOI: 10.1016/j.bbamcr.2007.09.005
Popis: Enteric neural crest cells (NCC) are multipotent progenitors which give rise to neurons and glia of the enteric nervous system (ENS) during fetal development. Glial cell line-derived neurotrophic factor (GDNF)/RET receptor tyrosine kinase (Ret) signaling is indispensable for their survival, migration and differentiation. Using microarray analysis and isolated NCCs, we found that 45 genes were differentially expressed after GDNF treatment (16 h), 29 of them were up-regulated including 8 previously undescribed genes. Prokineticin receptor 1 (PK-R1), a receptor for Prokineticins (Prok), was identified in our screen and shown to be consistently up-regulated by GDNF in enteric NCCs. Further, PK-R1 was persistently expressed at a lower level in the enteric ganglions of the c-Ret deficient mice when compared to that of the wild-type littermates. Subsequent functional analysis showed that GDNF potentiated the proliferative and differentiation effects of Prok-1 by up-regulating PK-R1 expression in enteric NCCs. In addition, expression analysis and gene knock-down experiments indicated that Prok-1 and GDNF signalings shared some common downstream targets. More importantly, Prok-1 could induce both proliferation and expression of differentiation markers of c-Ret deficient NCCs, suggesting that Prok-1 may also provide a complementary pathway to GDNF signaling. Taken together, these findings provide evidence that Prok-1 crosstalks with GDNF/Ret signaling and probably provides an additional layer of signaling refinement to maintain proliferation and differentiation of enteric NCCs.
Databáze: OpenAIRE
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