Targeting Antigen to Mouse Dendritic Cells via Clec9A Induces Potent CD4 T Cell Responses Biased toward a Follicular Helper Phenotype
| Autor: | Wei Shi, Chin-Nien Lee, Irina Caminschi, Susie Kitsoulis, Scott N. Mueller, Andrew M. Lew, Ken Shortman, Soo San Wan, Gordon K. Smyth, David Vremec, Fatma Ahmet, Belinda Phipson, Yu Kato, Mireille H. Lahoud, Gayle M. Davey, William R. Heath |
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| Rok vydání: | 2011 |
| Předmět: |
CD4-Positive T-Lymphocytes
Recombinant Fusion Proteins medicine.medical_treatment Immunology Antigen presentation Mice Transgenic Receptors Cell Surface Biology Immunophenotyping Minor Histocompatibility Antigens Mice Adjuvants Immunologic Antigen Antigens CD Vaccines DNA medicine Animals Humans Immunology and Allergy Cytotoxic T cell Lectins C-Type Receptors Immunologic Antigen Presentation Mice Inbred C3H Follicular dendritic cells Cross-presentation Dendritic Cells Cytotoxicity Tests Immunologic Mice Mutant Strains Cell biology Mice Inbred C57BL Receptors Mitogen Humoral immunity Adjuvant CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | The Journal of Immunology. 187:842-850 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.1101176 |
| Popis: | Three surface molecules of mouse CD8+ dendritic cells (DCs), also found on the equivalent human DC subpopulation, were compared as targets for Ab-mediated delivery of Ags, a developing strategy for vaccination. For the production of cytotoxic T cells, DEC-205 and Clec9A, but not Clec12A, were effective targets, although only in the presence of adjuvants. For Ab production, however, Clec9A excelled as a target, even in the absence of adjuvant. Potent humoral immunity was a result of the highly specific expression of Clec9A on DCs, which allowed longer residence of targeting Abs in the bloodstream, prolonged DC Ag presentation, and extended CD4 T cell proliferation, all of which drove highly efficient development of follicular helper T cells. Because Clec9A shows a similar expression pattern on human DCs, it has particular promise as a target for vaccines of human application. |
| Databáze: | OpenAIRE |
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