Carbocyanine dyes inhibit Ca-dependent K efflux from human red cell ghosts
| Autor: | T. J. B. Simons |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Membrane potential
chemistry.chemical_classification Erythrocytes Multidisciplinary K efflux Chemistry Potassium Erythrocyte Membrane Iodide chemistry.chemical_element Biological Transport Carbocyanines Calcium Inhibitory postsynaptic potential Fluorescence Biochemistry Quinolines Humans Coloring Agents |
| Zdroj: | Nature. 264:467-469 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/264467a0 |
| Popis: | CARBOCYANINE dyes are fluorescent probes of membrane potential in red cells1,2, and their application has been extended to Ehrlich ascites cells3,4 and phytoplankton5. I have used them to study the effect of potassium concentration gradients on the membrane potential of resealed ghosts containing Ca buffers. Intracellular Ca specifically renders these ghosts highly permeable to K (ref. 6). During this work, I discovered that 3,3′-dipropyl- and 3,3′-diethyl-thiadicarbocyanine iodide cause a progressive inhibition of K efflux at low external K concentrations, and that external K protects against the inhibitory effects of the dyes. The results suggest that the carbocyanine dyes are specific inhibitors of the Ca-dependent K transport system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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