Tyrosine phosphorylation is essential for DSCAML1 to promote dendrite arborization of mouse cortical neurons
| Autor: | Guoxin Jin, Jingzhu Duan, Xu Hou, Limin Lao, Shaoqian Cui |
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| Rok vydání: | 2013 |
| Předmět: |
Primary Cell Culture
Fasciculation Mice DSCAM chemistry.chemical_compound PAK1 medicine Animals Phosphorylation Cells Cultured Cerebral Cortex Neurons Cell adhesion molecule Chemistry General Neuroscience Tyrosine phosphorylation Dendrites Dendritic filopodia Cell biology Mice Inbred C57BL DOWN SYNDROME CELL ADHESION MOLECULE-LIKE 1 Animals Newborn nervous system Gene Knockdown Techniques Mutation Tyrosine medicine.symptom Cell Adhesion Molecules Neuroscience |
| Zdroj: | Neuroscience Letters. 555:193-197 |
| ISSN: | 0304-3940 |
| Popis: | Dendritic self-avoidance is critical for appropriate dendrite arborization. We herein examined the role of Down syndrome cell adhesion molecule like-1 (DSCAML1) in regulating dendritic self-avoidance and that of tyrosine phosphorylation in mediating the effects of DSCAML1. Knocking down DSCAML1 in newborn mouse cortical neurons compromised dendritic self-avoidance as evidenced by dendritic fasciculation and increased dendritic self-crossing. Introduction of a DSCAML1Y1808F mutant into the DSCAML1-knocked down neurons failed to reverse the abnormal dendritic arborization. These results suggest that DSCAML1 promotes dendritic self-avoidance in cortical neurons, and that phosphorylation at Y1808 is essential in mediating the effects of DSCAML1. |
| Databáze: | OpenAIRE |
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