In vivo measurement of lung preservation solution efficacy: comparison of LPD, UW, EC and low K+-EC following short and extended ischemia
| Autor: | Schroeder F, Hans-Joachim Schäfers, Bernard Hausen, Beuke M, Poets Cf, DelRossi Aj, Hewitt C |
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| Rok vydání: | 1998 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male medicine.medical_specialty Adenosine Time Factors Allopurinol Hypertonic Solutions Organ Preservation Solutions Urology Ischemia Raffinose In vivo hemic and lymphatic diseases medicine Animals Insulin Viaspan Lung Phospholipids business.industry Proteins Dextrans General Medicine medicine.disease Glutathione Surgery Rats Transplantation medicine.anatomical_structure Rats Inbred Lew Breathing Vascular resistance Potassium Cardiology and Cardiovascular Medicine business Perfusion Bronchoalveolar Lavage Fluid Lung Transplantation |
| Zdroj: | European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery. 12(5) |
| ISSN: | 1010-7940 |
| Popis: | Objective: The impact of storage solution composition on graft performance was evaluated following perfusion with either Euro-Collins (EC), low potassium Euro-Collins (rEC), low potassium dextran (LPD) or University of Wisconsin solution (UW) after brief (2 h) and extended ischemia (16 h) in an acute double lung transplantation model in the rat. Methods: Following flush perfusion and ischemia the lungs were implanted in recipient rats allowing serial assessment of graft pulmonary vascular resistance (PVR) and alveolar arterial oxygen difference (AaDO2) during 120 min of reperfusion. Graft dynamic lung compliance (DLC) was determined by separate ventilation. Final evaluation included weight gain and histology. Results: After extended ischemia LPD provided superior graft function in respect to DLC (repeated measures ANOVA; LPD versus rEC PB 0.05; versus EC PB0.03; versus UW PB0.05) and AaDO2 (LPD versus rEC PB 0.04; versus EC PB 0.006). The PVR was significantly lower in LPD versus UW (PB0.05). At the end of reperfusion the weight increase amounted to 2299 49% in rEC, 2079 22% in EC, 115922% in UW and 87917% in LPD (LPD versus rEC PB0.01, LPD versus EC PB 0.001). The type of preservation solution used had little impact on graft function after 2 h ischemia. Conclusions: Low potassium dextran provides superior graft function after extended ischemia. After short ischemia the type of preservation solution used in this study had little impact on global lung function. © 1997 Elsevier Science B.V. |
| Databáze: | OpenAIRE |
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