Molecular characterization of macrolide resistant Streptococcus pyogenes isolates from pharyngitis patients in Serbia
| Autor: | Ina Gajic, Anna L. Borek, Ivana Lazarevic, Natasa Opavski, Katarzyna Obszańska, Vera Mijac, Lazar Ranin, Maja Stanojevic, Izabela Sitkiewicz |
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| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Genotype Streptococcus pyogenes Virulence Factors Population Microbial Sensitivity Tests Multiple Loci VNTR Analysis Biology medicine.disease_cause Microbiology Group A 03 medical and health sciences Tandem repeat Streptococcal Infections Drug Resistance Bacterial Genetics medicine Cluster Analysis Humans education Molecular Biology Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences education.field_of_study Molecular epidemiology Virulence 030306 microbiology Pharyngitis 3. Good health Infectious Diseases Phenotype Genes Bacterial Multilocus sequence typing Macrolides Serbia Multilocus Sequence Typing |
| Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 33 |
| ISSN: | 1567-7257 |
| Popis: | A steady increase in macrolide resistance in Streptococcus pyogenes, group A streptococci (GAS) was reported in Serbia during 2004–2009 (9.9%). However, there are no data on the molecular epidemiology of pharyngeal macrolide resistance GAS (MRGAS) isolates. Therefore, the aims of this first nationwide study were to examine the prevalence of macrolide resistance in Serbian GAS and to determine their resistance phenotypes, genotypes and clonal relationships. Overall 3893 non-duplicate pharyngeal S. pyogenes isolates from outpatients with GAS infection were collected throughout country during 2008 and 2009. Among 486 macrolide resistant pharyngeal isolates collected, 103 were further characterized. Macrolide resistance phenotypes and genotypes were determined by double-disk diffusion test and PCR, respectively. Strain relatedness was determined by emm typing, multilocus sequence typing (MLST), multilocus variable tandem repeat analysis (MLVA), phage profiling (PP) and virulence factor profiling (VFP). Overall, macrolide resistance among GAS isolates in Serbia was 12.5%. M phenotype was the most common (71.8%), followed by iMLS (18.4%) and cMLS (9.7%). Three clonal complexes – emm75/mefA/ST49, emm12/mefA/ST36 and emm77/ermA/tetO/ST63 comprised over 90% of the tested strains. Although MLVA, PP and VFP distinguished 10, 20 and 12 different patterns, respectively, cluster analysis disclosed only small differences between strains which belonged to the same emm/ST type. Our data indicate dominance of three major internationally widely disseminated macrolide resistant clones and a high genetic homogeneity among the Serbian MRGAS population. Continued surveillance of macrolide resistance and clonal composition in MRGAS in Serbia in future is necessary to determine stability of MRGAS clones and to guide therapy strategies. |
| Databáze: | OpenAIRE |
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