BSN (bassoon) and PRKN/parkin in concert control presynaptic vesicle autophagy
| Autor: | Sheila Hoffmann-Conaway, Anil Annamneedi, Craig C. Garner, Eckart D. Gundelfinger, Carolina Montenegro-Venegas |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Scaffold protein Ubiquitin-Protein Ligases Presynaptic Terminals Nerve Tissue Proteins Synaptic vesicle Parkin Presynapse ultrastructure [Synaptic Vesicles] Mice 03 medical and health sciences metabolism [Ubiquitin-Protein Ligases] ddc:570 Autophagy Animals Active zone Molecular Biology metabolism [Synaptic Vesicles] metabolism [Nerve Tissue Proteins] 030102 biochemistry & molecular biology biology ultrastructure [Autophagosomes] Autophagosomes Cell Biology ultrastructure [Presynaptic Terminals] metabolism [Autophagosomes] Autophagic Punctum Cell biology Ubiquitin ligase 030104 developmental biology Proteostasis metabolism [Presynaptic Terminals] biology.protein Synaptic Vesicles |
| Zdroj: | Autophagy Autophagy 16(9), 1732-1733 (2020). doi:10.1080/15548627.2020.1801259 |
| ISSN: | 1554-8635 1554-8627 |
| Popis: | Maintaining the integrity and function of the presynaptic neurotransmitter release apparatus is a demanding process for a post-mitotic neuron; the mechanisms behind it are still unclear. BSN (bassoon), an active zone scaffolding protein, has been implicated in the control of presynaptic macroautophagy/autophagy, a process we recently showed depends on poly-ubiquitination of synaptic proteins. Moreover, loss of BSN was found to lead to smaller synaptic vesicle (SV) pools and younger pools of the SV protein SV2. Of note, the E3 ligase PRKN/parkin appears to be involved in BSN deficiency-related changes in autophagy levels, as shRNA-mediated knockdown of PRKN counteracts BSN-deficiency and rescues decreased SV protein levels as well as impaired SV recycling in primary cultured neurons. These data imply that BSN and PRKN act in concert to control presynaptic autophagy and maintain presynaptic proteostasis and SV turnover at the physiologically required levels. |
| Databáze: | OpenAIRE |
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