BMP signals regulate Dlx5 during early avian skull development
| Autor: | Anne-H.élène Monsoro-Burq, Alexandra Quilhac, Nicolas Holleville, Martine Bontoux |
|---|---|
| Přispěvatelé: | Laboratoire d'embryologie cellulaire et moléculaire (LECM), Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), Adaptation et evolution des sytemes osteo-musculaires, Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Muséum national d'Histoire naturelle (MNHN), Department of Molecular and Cellular Biology, Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
MESH: Signal Transduction
MESH: Bone Morphogenetic Proteins [SDV]Life Sciences [q-bio] Goosecoid FGF4 Gene mutation MESH: MSX1 Transcription Factor Mesoderm 0302 clinical medicine Cranial vault MESH: Gene Expression Regulation Developmental MESH: Animals 0303 health sciences MESH: Mesoderm FGFR Gene Expression Regulation Developmental MESH: Transcription Factors DLX5 MESH: Chick Embryo MESH: Goosecoid Protein Cell biology Bone morphogenetic protein 7 medicine.anatomical_structure MESH: Repressor Proteins Intramembranous ossification Bone Morphogenetic Proteins embryonic structures MESH: Cell Division MESH: Skull Cell Division MESH: Fibroblast Growth Factors Signal Transduction medicine.medical_specialty animal structures Mesenchyme Biology 03 medical and health sciences MESH: Gene Expression Profiling Noggin Internal medicine MESH: Homeodomain Proteins medicine Animals BMP Dlx5 Transcription factor Molecular Biology 030304 developmental biology Homeodomain Proteins MSX1 Transcription Factor MESH: Osteoblasts Osteoblasts Gene Expression Profiling Skull Cell Biology Fibroblast Growth Factors Repressor Proteins Goosecoid Protein Endocrinology Chick embryo 030217 neurology & neurosurgery Msx1 Developmental Biology Transcription Factors |
| Zdroj: | Developmental Biology Developmental Biology, Elsevier, 2003, 257 (1), pp.177-89. ⟨10.1016/S0012-1606(03)00059-9⟩ Developmental Biology, 2003, 257 (1), pp.177-89. ⟨10.1016/S0012-1606(03)00059-9⟩ |
| ISSN: | 0012-1606 1095-564X |
| DOI: | 10.1016/S0012-1606(03)00059-9⟩ |
| Popis: | International audience; The vertebrate skull vault forms almost entirely by the direct mineralisation of mesenchyme, without the formation of a cartilaginous template, a mechanism called membranous ossification. Dlx5 gene mutation leads to cranial dismorphogenesis which differs from the previously studied craniosynostosis syndromes [Development 126 (1999), 3795; Development 126 (1999), 3831]. In avians, little is known about the genetic regulation of cranial vault development. In this study, we analyze Dlx5 expression and regulation during skull formation in the chick embryo. We compare Dlx5 expression pattern with that of several genes involved in mouse cranial suture regulation. This provides an initial description of the expression in the developing skull of the genes encoding the secreted molecules BMP 2, BMP 4, BMP 7, the transmembrane FGF receptors FGFR 1, FGFR 2, FGFR 4, the transcription factors Msx1, Msx2, and Twist, as well as Goosecoid and the early membranous bone differentiation marker osteopontin. We show that Dlx5 is activated in proliferating osteoblast precursors, before osteoblast differentiation. High levels of Dlx5 transcripts are observed at the osteogenic fronts (OFs) and at the edges of the suture mesenchyme, but not in the suture itself. Dlx5 expression is initiated in areas where Bmp4 and Bmp7 genes become coexpressed. In a calvarial explant culture system, Dlx5 transcription is upregulated by BMPs and inhibited by the BMP-antagonist Noggin. In addition, FGF4 activates Bmp4 but not Bmp7 gene transcription and is not sufficient to induce ectopic Dlx5 expression in the immature calvarial mesenchyme. From these data, we propose a model for the regulatory network implicated in early steps of chick calvarial development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect