Comparison of the automated fluorescence microscopic viability test with the conventional and flow cytometry methods
| Autor: | Dae Sung Hur, Chae Seung Lim, Jun Koon Chang, Chanil Chung, Jang Su Kim, Seong Soo A. An, Myung Hyun Nam |
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| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Cell Membrane Permeability Cell Survival Clinical Biochemistry Analytical chemistry Diagnostic tools Stain Flow cytometry Leukocyte Count chemistry.chemical_compound Lab-On-A-Chip Devices medicine Humans Immunology and Allergy Viability assay Propidium iodide Chromatography medicine.diagnostic_test Chemistry Biochemistry (medical) Public Health Environmental and Occupational Health Reproducibility of Results Original Articles Trypan Blue Hematology Flow Cytometry Fluorescence Running time Medical Laboratory Technology Microscopy Fluorescence Leukocytes Mononuclear Trypan blue |
| Zdroj: | Journal of Clinical Laboratory Analysis. 25:90-94 |
| ISSN: | 0887-8013 |
| Popis: | The cell viability test is an essential tool in any laboratory, performing cell‐based studies and clinical laboratory tests. The trypan blue exclusion method is the most popular assay for its simple concept among various diagnostic tools. However, several disadvantages include time‐consuming and labor‐intensive steps with low precision. In this study, we evaluated a new technique for the automatic cell viability measurement with microscopic cell counter and microchip. Upon blood draw from 11 healthy volunteers, Mononuclear cells were separated immediately from the heparinized whole blood, and the viable cells were diluted from 100 to 1%. The cell viability tests were performed simultaneously with following three methods: the conventional manual trypan blue exclusion method; the flow cytometry measurement with propidium iodide stain; and the newly developed microscopic cell counter with microchip. Linearities, precisions, and correlations from three methods were analyzed and compared. The correlations data from the microscopic cell counter were in good agreement with both the conventional trypan blue method (r=0.99, P |
| Databáze: | OpenAIRE |
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