Transcriptome-wide identification of differentially expressed genes in Procambarus clarkii in response to chromium challenge
| Autor: | Xun Meng, Wei-Wei Zhang, Liang Hong, Yu Liu, Ting Jiao, Dai-Zhen Zhang, Qiu-Ning Liu, Bo-Ping Tang, Xiao-Hua Chu, Wen-Fei He, Ting-Ting Yang, Jia-Lian Wang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Potassium Compounds Astacoidea Aquatic Science Antioxidants Transcriptome 03 medical and health sciences Chromates Environmental Chemistry Animals Gene Shellfish Procambarus clarkii chemistry.chemical_classification biology Gene Expression Profiling RNA 04 agricultural and veterinary sciences General Medicine biology.organism_classification Crayfish 030104 developmental biology Enzyme chemistry Biochemistry Catalase 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Water Pollutants Chemical |
| Zdroj: | Fishshellfish immunology. 87 |
| ISSN: | 1095-9947 |
| Popis: | Because of the high protein content and rich meat quality of crayfish Procambarus clarkii, it has become widely popular in China in recent years and has a high economic value. When P. clarkii is stimulated by heavy metals, it reacts to oxidation. P. clarkii has evolved antioxidant defense systems, including antioxidant enzymes such as catalase (CAT). The hexavalent form of Cr (VI) is a pathogenic factor that is of particular concern in aqueous systems because of its great toxicity to living organisms. In this study, we characterized the transcriptome of P. clarkii using a RNA sequencing method and performed a comparison between K2Cr2O7-treated samples and controls. In total, 34,237 unigenes were annotated. We identified 5098 significantly differentially expressed genes (DEGs), including 2536 and 2562 were significantly up-regulated and down-regulated, respectively. In addition, quantitative real time-PCR (qRT-PCR) confirmed the up-regulation of a random selection of DEGs. Our results contribute to a more comprehensive understanding of the antioxidant defense system used by P. clarkii in response to heavy metal stress. |
| Databáze: | OpenAIRE |
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