High density DNA data storage library via dehydration with digital microfluidic retrieval
| Autor: | Ashley P. Stephenson, Sharon Newman, Luis Ceze, Karin Strauss, Bichlien H. Nguyen, Christopher N. Takahashi, Max Willsey |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Computer science Science DNA digital data storage Microfluidics General Physics and Astronomy High density Information Storage and Retrieval 02 engineering and technology Data loss General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Lab-On-A-Chip Devices Digital microfluidics Hardware_ARITHMETICANDLOGICSTRUCTURES Desiccation lcsh:Science Multidisciplinary Spots business.industry Computational Biology Water General Chemistry DNA Equipment Design 021001 nanoscience & nanotechnology 030104 developmental biology Scalability Computer data storage lcsh:Q Glass 0210 nano-technology Biological system business |
| Zdroj: | Nature Communications Nature Communications, Vol 10, Iss 1, Pp 1-6 (2019) |
| ISSN: | 2041-1723 |
| Popis: | DNA promises to be a high density data storage medium, but physical storage poses a challenge. To store large amounts of data, pools must be physically isolated so they can share the same addressing scheme. We propose the storage of dehydrated DNA spots on glass as an approach for scalable DNA data storage. The dried spots can then be retrieved by a water droplet using a digital microfluidic device. Here we show that this storage schema works with varying spot organization, spotted masses of DNA, and droplet retrieval dwell times. In all cases, the majority of the DNA was retrieved and successfully sequenced. We demonstrate that the spots can be densely arranged on a microfluidic device without significant contamination of the retrieval. We also demonstrate that 1 TB of data could be stored in a single spot of DNA and successfully retrieved using this method. DNA as a high density storage medium is receiving increasing attention, but long term physical storage is an unsolved problem. Here the authors show that up to 1 TB of data stored as dehydrated DNA spots on a glass cartridge can be retrieved in a spot of water using digital microfluidics with minimal data loss and contamination. |
| Databáze: | OpenAIRE |
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