A phenothiazine coumarin based ratiometric fluorescent probe for real-time detection of lysosomal hypochlorite in living cell and zebra fish
| Autor: | Jiaojiao Liu, Liuhe Wei, Peixin Niu, Yifan Rong, Wenqiang Chen, Xingjiang Liu, Xiangzhi Song |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Hypochlorite
02 engineering and technology Living cell 010402 general chemistry 01 natural sciences Analytical Chemistry chemistry.chemical_compound Coumarins Phenothiazines Phenothiazine Lysosome medicine Animals Instrumentation Spectroscopy Zebrafish Fluorescent Dyes Detection limit chemistry.chemical_classification Reactive oxygen species 021001 nanoscience & nanotechnology Coumarin Fluorescence Atomic and Molecular Physics and Optics 0104 chemical sciences Hypochlorous Acid medicine.anatomical_structure chemistry Biophysics 0210 nano-technology Lysosomes |
| Zdroj: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 261 |
| ISSN: | 1873-3557 |
| Popis: | Hypochlorite (ClO-), a type of reactive oxygen species (ROS), plays an essential role in complex biological systems. Real-time detection of the content and distribution of ClO- in cells or subcellular organelle is critically essential. In this paper, a lysosomal-targeted fluorescent probe, Cou-Lyso, was constructed for real-time detection of ClO- in a ratiometric manner, achieving high sensitivity with a low detection limit (0.58 μM). Upon reaction with ClO-, this probe was subjected to a significant fluorescence change from red emission (λmaxem = 610 nm) to green emission (λmaxem = 535 nm) with the ratio of I535 nm/I610 nm displaying a 76-fold enhancement from 0.04 to 3.03. The confocal imaging experiments for Cou-Lyso showed that this probe could detect ClO- in living cell and zebra fish. This probe has been successfully applied to stain lysosome and image lysosomal ClO- based on co-localization imaging experiments. |
| Databáze: | OpenAIRE |
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