Protein Layer on Hemodialysis Membranes: A New Immunohistochemistry Technique
| Autor: | S De Pietro, A. M. Bianchi, Vincenzo Panichi, Roberto Palla, V Gattai, L. Casarosa, B. Andreini, Luca Giovannini, Massimiliano Migliori |
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| Rok vydání: | 1995 |
| Předmět: |
Frozen section procedure
Pathology medicine.medical_specialty Chromatography biology Chemistry Antithrombin 030232 urology & nephrology Biomedical Engineering Medicine (miscellaneous) Bioengineering General Medicine 030204 cardiovascular system & hematology Fibrinogen Fluorescence Biomaterials 03 medical and health sciences 0302 clinical medicine Membrane medicine biology.protein Immunohistochemistry Factor XIIIa Antibody medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1724-6040 0391-3988 |
| Popis: | In order to investigate the nature of the protein layer deposited on hemodialysis membranes we developed a direct immunohistochemical technique using fluoresceinated antibodies to plasma proteins. Fifteen patients on regular dialytic treatment were dialyzed with CU, HE, PAN, PS and PMMA and the dialyzers analyzed at the end of a standard dialytic session. Snap frozen sections of hollow fiber devices were treated with flourescein-isothiocyanate conjugated antibodies for IgG, IgA, IgM, C3c, fibrinogen, factor VIII, factor Xllla-s, antithrombin III, fibrinogen degradation products (PDF) and plasminogen. Protein deposits were evaulated by a quantitative criteria, which evaluates the intensity of fluorescence and the area of the capillary wall occupied by this fluorescence by using an image analysis software. The coagulation cascade is activated by all membranes and similar deposits of these proteins were revealed, whereas important differences in C3c deposition were found. |
| Databáze: | OpenAIRE |
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