Stimulation of intermolecular ligation withE. coliDNA ligase by high concentration of monovalent cations in polyethylene glycol solutions
| Autor: | Nobutsugu Hiraoka, Yukuo Ishizaki, Masako Nakazawa, Akira Obayashi, Ken-ichiro Hayashi |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
DNA
Bacterial DNA Ligases Sodium Potassium Salt (chemistry) chemistry.chemical_element Polyethylene glycol Biology medicine.disease_cause Polyethylene Glycols chemistry.chemical_compound Bacterial Proteins PEG ratio Escherichia coli Genetics medicine chemistry.chemical_classification DNA ligase Molecular biology Adenosine Monophosphate Molecular Weight Polynucleotide Ligases chemistry DNA Circular Ligation Plasmids Nuclear chemistry |
| Zdroj: | Nucleic Acids Research. 13:7979-7992 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/13.22.7979 |
| Popis: | In the presence of high concentrations of the nonspecific polymer polyethylene glycol (PEG), intermolecular cohesive-end ligation with the DNA ligase from Escherichia coli was stimulated by high salt concentrations: 200 mM NaCl or 300 mM KCl in 10% (w/v) PEG 6000 solutions, and 100-200 mM NaCl or 150-300 mM KCl in 15% PEG 6000 solutions. Intermolecular blunt-end ligation with this ligase was also stimulated at 100-150 mM NaCl or 150-250 mM KCl in 15% PEG 6000 solutions. The extent of such intermolecular ligation increased and the salt concentrations at which ligation was stimulated extended to lower concentrations when we raised the temperature from 10 to 37 degrees C. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|