Increased adipose tissue PC-1 protein content, but not tumour necrosis factor-a gene expression, is associated with a reduction of both whole body insulin sensitivity and insulin receptor tyrosine-kinase activity
| Autor: | Riccardo Vigneri, Jack F. Youngren, Monica D’Adamo, P. Sbraccia, A. Buongiorno, I. D. Goldfine, Lucia Frittitta, Vincenzo Trischitta |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Endocrinology Diabetes and Metabolism medicine.medical_treatment Gene Expression Adipose tissue Enzyme-Linked Immunosorbent Assay Biology Internal medicine Internal Medicine medicine Humans Insulin Pyrophosphatases Pancreatic hormone Analysis of Variance Membrane Glycoproteins Phosphoric Diester Hydrolases Tumor Necrosis Factor-alpha Insulin tolerance test Skeletal muscle Middle Aged Receptor Insulin Kinetics Insulin receptor Endocrinology Cytokine medicine.anatomical_structure Adipose Tissue Injections Intravenous biology.protein Female Tumor necrosis factor alpha |
| Zdroj: | Diabetologia. 40:282-289 |
| ISSN: | 1432-0428 0012-186X |
| DOI: | 10.1007/s001250050675 |
| Popis: | In the present study we measured PC-1 content, tumour necrosis factor (TNF)-α gene expression, and insulin stimulation of insulin receptor tyrosine-kinase activity in adipose tissue from non-obese, non-diabetic subjects. These parameters were correlated with in vivo insulin action as measured by the intravenous insulin tolerance test (Kitt values). PC-1 content was negatively correlated with Kitt values (r = –0.5, p = 0.04) and positively with plasma insulin levels both fasting (r = 0.58, p = 0.009) and after 120 min during oral glucose tolerance test (OGTT) (r = 0.67, p = 0.002). Moreover, adipose tissue PC-1 content was higher in relatively insulin-resistant subjects (Kitt values lower than 6) than in relatively insulin-sensitive subjects (Kitt values higher than 6) (525 ± 49 ng/mg protein vs 336 ± 45, respectively, p = 0.012). Adipose tissue insulin receptor tyrosine-kinase activity in response to insulin was significantly lower at all insulin concentrations tested (p = 0.017, by two-way analysis of variance test) in insulin-resistant than in insulin-sensitive subjects (Kitt values lower or higher than 6, respectively). In contrast to PC-1, no significant correlation was observed between adipose tissue TNF-α mRNA content and Kitt values, and plasma insulin levels, both fasting and at after 120 min during OGTT. Also, no difference was observed in TNF-α mRNA content between subjects with Kitt values higher or lower than 6. These studies in adipose tissue, together with our previous studies in skeletal muscle raise the possibility that PC-1, by regulating insulin receptor function, may play a role in the degree of insulin sensitivity in non-obese, non-diabetic subjects. [Diabetologia (1997) 40: 282–289] |
| Databáze: | OpenAIRE |
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