Comparison of immunofluorescence with enzyme immunoassay for detection of Q fever
| Autor: | V. Cascante Burgos, S. Cruchaga D'Harcourt, D. Lozano Calero, A. Buño Soto, R. Martínez-Zapico |
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| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
Q fever Biology Immunofluorescence Sensitivity and Specificity Immunoglobulin G Immunoenzyme Techniques parasitic diseases medicine Humans Fluorescent Antibody Technique Indirect chemistry.chemical_classification medicine.diagnostic_test virus diseases General Medicine bacterial infections and mycoses medicine.disease Antibodies Bacterial Virology Titer Infectious Diseases Enzyme Rickettsiosis chemistry Coxiella burnetii Immunoassay biology.protein Antibody Q Fever |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 15:749-752 |
| ISSN: | 1435-4373 0934-9723 |
| DOI: | 10.1007/bf01691965 |
| Popis: | To evaluate enzyme immunoassay (EIA) as an alternative to indirect immunofluorescence assay (IFA) to screen for Q fever in humans, 157 serum samples from patients suspected of having the disease were tested for immunoglobulin G antibodies toCoxiella burnetii. The agreement between the tests and the sensitivity of EIA were excellent (96.8% and 98.4%, respectively) when an IFAtiter of > 1/160 was considered positive. All serum samples with a titer of > 1/320 in the IFA were also positive by the EIA. The EIA seems to be an acceptable alternative to IFA for screening for Q fever. |
| Databáze: | OpenAIRE |
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