Clearance of platelet microparticles in vivo
| Autor: | Bettina Toth, R. Pihusch, Andreas Rank, M. Iberer, Alexander Crispin, S. Grützner, Rienk Nieuwland |
|---|---|
| Přispěvatelé: | ACS - Amsterdam Cardiovascular Sciences, CCA -Cancer Center Amsterdam, Laboratory Specialized Diagnostics & Research |
| Rok vydání: | 2011 |
| Předmět: |
Adult
Blood Platelets Male Adolescent Platelet Transfusion Pharmacology Flow cytometry Young Adult Annexin In vivo Antigens CD Cell-Derived Microparticles Medicine Humans Platelet Platelet activation Annexin A5 Aged medicine.diagnostic_test business.industry Platelet Count Hematology General Medicine Middle Aged Kinetics P-Selectin Apheresis Platelet transfusion Immunology Female business CD61 Half-Life |
| Zdroj: | Platelets, 22(2), 111-116. Informa Healthcare |
| ISSN: | 1369-1635 0953-7104 |
| Popis: | At present, little is known about the clearance of platelet-derived microparticles (PMP) in human blood, as due to ethical considerations infusion experiments with labeled microparticles are delicate. Therefore, we investigated the kinetics of PMP, which are abundantly present in apheresis platelet concentrates (PC), following platelet transfusion in severe thrombocytopenic patients (n?=?11). PMP were double-stained with annexin V and cell-specific antibodies (anti-CD61, anti-CD63 or anti-CD62P, respectively) and detected by flow cytometry before and after transfusion of a single PC at fixed time intervals. Upon transfusion, the plasma levels of MP binding annexin V (2.5-fold), PMP (CD61+; 2.9-fold), and PMP from activated platelets (CD63+; 1.9-fold) or P-selectin (2.5-fold) increased immediately. The plasma levels of MP decreased with a half life of 5.8 hours (annexin V; 95% CI: 1.8?18.3) and 5.3 hours (CD61; 95% CI: 2.0?14.2). This is the first report in which the half life time of transfused PMP has been investigated in humans |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|