Genetic identity of aminoglycoside-resistance genes in Escherichia coli isolates from human and animal sources
| Autor: | Kin-Hung Chow, Stephanie W. Lo, Samson S. Y. Wong, Pak-Leung Ho, River C. W. Wong, Tak-Lun Que |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
DNA Bacterial Microbiology (medical) Drug resistance Biology medicine.disease_cause Microbiology Plasmid Acetyltransferases Drug Resistance Bacterial Escherichia coli medicine Pulsed-field gel electrophoresis Animals Cluster Analysis Humans Child Gene Alleles Escherichia coli Infections Genetics Genetic diversity Molecular epidemiology Animal Sources Escherichia coli Proteins Genetic Variation Sequence Analysis DNA General Medicine DNA Fingerprinting Anti-Bacterial Agents Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Aminoglycosides Genes Bacterial Child Preschool Hong Kong Female Plasmids |
| Zdroj: | Journal of Medical Microbiology. 59:702-707 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.015032-0 |
| Popis: | A bacterial collection (n=249) obtained in Hong Kong from 2002 to 2004 was used to investigate the molecular epidemiology of aminoglycoside resistance among Escherichia coli isolates from humans and food-producing animals. Of these, 89 isolates were gentamicin-sensitive (human n=60, animal n=29) and 160 isolates were gentamicin-resistant (human n=107, animal n=53). Overall, 84.1 % (90/107) and 75.5 % (40/53) of the gentamicin-resistant isolates from human and animal sources, respectively, were found to possess the aacC2 gene. The aacC2 gene for 20 isolates (10 each for human and animal isolates) was sequenced. Two alleles were found that were equally distributed in human and animal isolates. PFGE showed that the gentamicin-resistant isolates exhibited diverse patterns with little clonality. In some isolates, the aacC2 gene was encoded on large transferable plasmids of multiple incompatibility groups (IncF, IncI1 and IncN). An IncFII plasmid of 140 kb in size was shared by one human and three animal isolates. In summary, this study showed that human and animal isolates share the same pool of resistance genes. |
| Databáze: | OpenAIRE |
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