Differential abundances of four forms of binder of SPerm 1 in the seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability
Autor: | Marcos Jorge Magalhães, Leonardo Franco Martins, Denise Silva Okano, José Domingos Guimarães, Sérgio Campos, Alessandra C. Faria-Campos, Thaís Ferreira dos Santos, Renato Lima Senra, Maria Cristina Baracat-Pereira, Paulo Roberto Gomes Pereira |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Male endocrine system Semen cryopreservation medicine.medical_treatment Semen BSP1 Biology Seminal Vesicle Secretory Proteins Cryopreservation Proteomics Computational tools Andrology 03 medical and health sciences Nelore bulls fluids and secretions Human fertilization Food Animals Freezing medicine Animals Protein Isoforms Computational analysis Small Animals Protein spot urogenital system Equine Artificial insemination Sperm 030104 developmental biology Freezability Animal Science and Zoology Cattle Semen Preservation |
Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
Popis: | The Binder of SPerm 1 (BSP1) protein is involved in the fertilization and semen cryopreservation processes and is described to be both beneficial and detrimental to sperm. Previously, the relationship of BSP1 with freezability events has not been completely understood. The objective of this work was to determine the differential abundance of the forms of the BSP1 protein in cryopreserved seminal plasma of Bos taurus indicus bulls with different patterns of semen freezability using proteomics. A wide cohort of adult bulls with high genetic value from an artificial insemination center was used as donors of high quality, fresh semen. Nine bulls presenting different patterns of semen freezability were selected. Two-dimensional gel electrophoresis showed differential abundance in a group of seven protein spots in the frozen/thawed seminal plasma from the bulls, ranging from 15 to 17 kDa, with pI values from 4.6 to 5.8. Four of these spots were confirmed to be BSP1 using mass spectrometry, proteomics, biochemical, and computational analysis (Tukey's test at P |
Databáze: | OpenAIRE |
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