CD3D, GZMK, and KLRB1 Are Potential Markers for Early Diagnosis of Rheumatoid Arthritis, Especially in Anti-Citrullinated Protein Antibody-Negative Patients
| Autor: | Shi Huipeng, Wenxiu Duan, Yapeng Zhu, Jian Zhou, Junqin Lu, Yihui Bi |
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| Rok vydání: | 2021 |
| Předmět: |
musculoskeletal diseases
rheumatoid arthritis RM1-950 ACPA-negative Pathogenesis medicine Rheumatoid factor Pharmacology (medical) Original Research Pharmacology biology business.industry KLRB1 Anti–citrullinated protein antibody GZMK bioinformatics CD3D medicine.disease Omics Rheumatoid arthritis Immunology biology.protein Biomarker (medicine) biomarker Therapeutics. Pharmacology Antibody business |
| Zdroj: | Frontiers in Pharmacology Frontiers in Pharmacology, Vol 12 (2021) |
| ISSN: | 1663-9812 |
| Popis: | Early diagnosis and monitoring of rheumatoid arthritis (RA) progress are critical for effective treatment. In clinic, the detection of rheumatoid factor (RF) and anti-citrullinated protein antibodies (ACPA) are usually combined to diagnose early RA. However, the poor specificity of RF and high heterogeneity of ACPA make the early diagnosis of RA still challenging. Bioinformatics analysis based on high-throughput omics is an emerging method to identify novel and effective biomarkers, which has been widely used in many diseases. Herein, utilizing an integrated strategy based on expression correlation analysis and weighted gene coexpression network analysis (WGCNA), we identified 76 RA-trait different expression genes (DEGs). Combined with protein-protein interaction (PPI) network construction and clustering, new hub genes associated in RA synovia, CD3D, GZMK, and KLRB1, were identified. We verified the specificity of these genes in the synovium of RA patients through three external datasets. We also observed high sensitivity and specificity of them for ACPA-negative patients. CD3D, GZMK, and KLRB1 are potentially key mediators of RA pathogenesis and markers for RA diagnosis. |
| Databáze: | OpenAIRE |
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