Analysis of HIV-1 diversity, primary drug resistance and transmission networks in Croatia

Autor: Maja Oroz, Robert Beluzić, Oliver Vugrek, Josip Begovac, Tomaž Mark Zorec, Snježana Židovec Lepej, Maja M. Lunar, Ana Planinić, Filip Rokić, Petra Korać, Mario Poljak
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Croatia / epidemiology
Male
Epidemiology
Human immunodeficiency virus (HIV)
lcsh:Medicine
HIV Infections
Drug resistance
medicine.disease_cause
law.invention
HIV Infections / epidemiology
law
Genotype
Prevalence
Medicine
lcsh:Science
Hiv resistance
Phylogeny
Molecular Epidemiology
Multidisciplinary
HIV Infections / virology
biology
Molecular medicine
HIV-1 / isolation & purification
3. Good health
Integrase
Transmission (mechanics)
Female
Anti-HIV Agents / pharmacology
Anti-HIV Agents / therapeutic use
Adult
Drug Resistance
Viral / genetics
Anti-HIV Agents
Croatia
030106 microbiology
HIV
molekularna epidemiologija
transmisijski klasteri
filogenetska analiza
filodinamska analiza
sekvenciranje nove generacije
Article
03 medical and health sciences
HIV Infections / transmission
Drug Resistance
Viral
Humans
Clinical care
Molecular epidemiology
business.industry
lcsh:R
Virology
Molecular Typing
030104 developmental biology
HIV-1 / drug effects
Mutation
biology.protein
HIV-1
lcsh:Q
HIV-1 / genetics
business
Zdroj: Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
DOI: 10.1038/s41598-019-53520-8
Popis: Molecular epidemiology of HIV-1 infection in treatment-naive HIV-1 infected persons from Croatia was investigated. We included 403 persons, representing 92.4% of all HIV-positive individuals entering clinical care in Croatia in 2014–2017. Overall prevalence of transmitted drug resistance (TDR) was estimated at 16.4%. Resistance to nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside RTI (NNRTIs) and protease inhibitors (PIs) was found in 11.4%, 6.7% and 2.5% of persons, respectively. Triple-class resistance was determined in 2.2% of individuals. In addition, a single case (1.0%) of resistance to integrase strand-transfer inhibitors (InSTIs) was found. Deep sequencing was performed on 48 randomly selected samples and detected additional TDR mutations in 6 cases. Phylogenetic inference showed that 347/403 sequences (86.1%) were part of transmission clusters and identified forward transmission of resistance in Croatia, even that of triple-class resistance. The largest TDR cluster of 53 persons with T215S was estimated to originate in the year 1992. Our data show a continuing need for pre-treatment HIV resistance testing in Croatia. Even though a low prevalence of resistance to InSTI was observed, surveillance of TDR to InSTI should be continued.
Databáze: OpenAIRE
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