EPITOPES OF THE P-FIMBRIAL ADHESIN OF E. COLI CAUSE DIFFERENT URINARY TRACT INFECTIONS
| Autor: | James A. Roberts, M. Bernice Kaack, Gary Baskin, Staffan Normark, Britt-Inger Marklund |
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| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Journal of Urology. 158:1610-1613 |
| ISSN: | 1527-3792 0022-5347 |
| Popis: | Purpose: This is a study of the interaction of the tip protein of P-fimbriae E. coli, its specific urothelial adhesin, and urothelial receptors for the adhesin. This tip protein has several epitopes that adhere to different isoreceptors containing the urothelial alpha-gal-1-4 beta-gal disaccharide. Renal tubular cells of our monkey model contain the globoside isoreceptor, and thus ureteral inoculation of E. coli with the class II tip protein leads to pyelonephritis. The class III tip protein adheres to the Forssman antigen and causes cystitis in humans.Materials and Methods: An E. coli strain, DS17 which originally caused pyelonephritis in a child, is P-fimbriated and contains a class II tip adhesin. A mutant was produced to contain a class III tip adhesin. Eight monkeys had a ureteral inoculation of E. coli DS17 and 4 monkeys with E. coli DS17-1. In addition, we studied in vitro adherence by these strains.Results: We show that in vitro adherence by the tip protein of P-fimbriae to bladder cells o... |
| Databáze: | OpenAIRE |
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