Phenolic fingerprint of peppermint leaves
| Autor: | R.M. Seabra, Federico Ferreres, Filipe Areias, Paula B. Andrade, Patrícia Valentão |
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| Přispěvatelé: | Agência Nacional de Inovação (Portugal), Fundação para a Ciência e a Tecnologia (Portugal) |
| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 5 pages, 4 figures, 2 tables. A reversed-phase high-performance liquid chromatography procedure is proposed for the determination of 10 phenolic compounds (eriodictyol 7-O-rutinoside, eriodictyol 7-O-glucoside, luteolin 7-O-rutinoside, luteolin 7-O-glucoside, hesperetin 7-O-rutinoside, apigenin 7-O-rutinoside, rosmarinic acid, 5,6-dihydroxy-7,8,3′,4′-tetramethoxyflavone, pebrellin and gardenin B) in peppermint. The chromatographic separation was achieved using a reversed-phase Spherisorb ODS 2 (5 μm particle size; 25.0×0.46 cm) column. Of the several extractive solvents tried, ethanol was the best for qualitative and quantitative analysis. Best resolution was obtained using a gradient of water-phosphoric acid (999:1) and acetonitrile. Fourteen samples were subjected to quantification and showed a common composition pattern. The authors are grateful to Agência de Inovaçâo and Praxis XXI for financial support of this work. Filipe Areias is indebted to Fundaçâo para a Ciência e a Tecnologia (PRAXIS XXI/BIC/12251/97) for a grant. |
| Databáze: | OpenAIRE |
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