Use of transforming growth factor-β loaded onto β-tricalcium phosphate scaffold in a bone regeneration rat calvaria model
| Autor: | Hadar Zigdon-Giladi, Tal Tamari, Rina Elimelech, Nizar Khoury, Israel Blumenfeld, Zvi Gutmacher |
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| Rok vydání: | 2018 |
| Předmět: |
Calcium Phosphates
Scaffold Bone Regeneration 0206 medical engineering Calvaria 02 engineering and technology Transforming Growth Factor beta1 03 medical and health sciences 0302 clinical medicine In vivo Osteogenesis Transforming Growth Factor beta medicine Animals Bone regeneration General Dentistry Tissue Engineering Chemistry Mesenchymal stem cell Skull Cell Differentiation 030206 dentistry 020601 biomedical engineering In vitro Cell biology Rats Transplantation medicine.anatomical_structure Transforming Growth Factors Oral Surgery Transforming growth factor |
| Zdroj: | Clinical implant dentistry and related researchREFERENCES. 21(4) |
| ISSN: | 1708-8208 |
| Popis: | Background Transforming growth factor-β (TGF-β1 ) enhances mesenchymal stem cell (MSC) differentiation into osteoblasts. Purpose The aim of the study was to assess whether TGF-β1 loaded onto β-tricalcium phosphate (β-TCP) synthetic scaffold enhances bone regeneration in a rat calvaria model. The release kinetics of TGF-β1 from β-TCP scaffold was evaluated in vitro. Materials and methods TGF-β1 in various concentrations (1-40 ng/mL) was loaded onto the β-TCP scaffold, and release kinetics was monitored by ELISA. The effect of TGF-β1 on the proliferation of MSCs was assessed using AlamarBlue, and MSC differentiation was evaluated by Alizarin Red quantification assay.Bone augmentation following transplantation of TGF-β1 loaded onto β-TCP in a rat calvaria model was evaluated in vivo. Results Greater TGF-β1 release from the 40 ng/mL concentration was found. A suppressive effect of TGF-β on the MSCs proliferation was observed with maximum inhibition obtained with 40 ng/mL compared to the control group (P = .028). A positive effect on MSCs osteogenic differentiation was found.Bone height and bone area fraction in vivo were similar with or without TGF-β1 ; however, blood vessel density and degradation of the scaffold were significantly higher in the TGF-β1 group. Conclusion TGF-β1 adsorbed to β-TCP stimulated angiogenesis and scaffold degradation that may enhance bone formation. |
| Databáze: | OpenAIRE |
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