IL-31 does not induce normal human ciliated epithelial cells to differentiate into a phenotype consistent with the pathophysiology of asthma
| Autor: | Liam G Heaney, Michael E. McBrien, Grzegorz Skibinski, JC Parker, Michael D. Shields, Surendran Thavagnanam |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Pathophysiology of asthma
business.industry medicine.medical_treatment Immunology IL-31 Allergic asthma Atopic dermatitis medicine.disease Phenotype Article respiratory tract diseases body regions Pathogenesis Cytokine immune system diseases Bronchial epithelial cells Differentiation medicine Ciliated cells business Bronchial epithelium Goblet cells Asthma |
| Zdroj: | Results in Immunology. 2:104-111 |
| ISSN: | 2211-2839 |
| DOI: | 10.1016/j.rinim.2012.05.001 |
| Popis: | BackgroundIL-31 is a novel cytokine that has been implicated in allergic diseases such as atopic dermatitis and more recently asthma. While IL-31 has been well studied in skin conditions such as atopic dermatitis, little is known about the role IL-31 plays in asthma and specifically the differentiation process of the bronchial epithelium, which is central to the pathogenesis of allergic asthma.MethodsWe examined the effects of IL-13 (20ng/ml), IL-31 (20ng/ml) and an IL-13/IL-31 combination stimulation (20ng/ml each) on the in vitro mucociliary differentiation of paediatric bronchial epithelial cells (PBECs) from healthy patients (n=6). IL-31 receptor (IL-31-RA) expression, markers of differentiation (goblet and ciliated cells), transepithelial electrical resistance (TEER), quantification of goblet and ciliated cells, real time PCR for MUC5AC, ELISA for VEGF, EGF and MCP-1 (CCL-2) and ELISA for MUC5AC were assessed.ResultsWe found that well-differentiated PBECs expressed IL-31-RA however it's expression did not increase upon stimulation with IL-31 or either of the other treatments. TEER indicated good formation of tight junctions which was found to be similar across all treatment groups (p=0.9). We found that IL-13 alone significantly reduced the number of ciliated cells compared with unstimulated (IL-13 stimuation: mean=4.8% (SD=2.5); unstimulated: mean=15.9%, (SD=7.4), p |
| Databáze: | OpenAIRE |
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