Computational and biological characterization of fusion proteins of two insecticidal proteins for control of insect pests
| Autor: | Sehrish Naz, Shaista Javaid, Zaheer Ul-Haq, Georg Jander, Shahid Mansoor, Imran Amin |
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| Rok vydání: | 2018 |
| Předmět: |
Models
Molecular 0106 biological sciences 0301 basic medicine Insecticides Recombinant Fusion Proteins Nicotiana tabacum Spider Venoms lcsh:Medicine Genetically modified crops Ligands 01 natural sciences Article Protein Structure Secondary 03 medical and health sciences Protein structure Bacillus thuringiensis Arachnida Animals Pest Control Biological lcsh:Science Multidisciplinary biology lcsh:R fungi food and beverages Lectin Hydrogen Bonding biology.organism_classification Potato virus X Hadronyche versuta Fusion protein 030104 developmental biology Biochemistry biology.protein lcsh:Q 010606 plant biology & botany |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-23138-3 |
| Popis: | Sucking pests pose a serious agricultural challenge, as available transgenic technologies such as Bacillus thuringiensis crystal toxins (Bt) are not effective against them. One approach is to produce fusion protein toxins for the control of these pests. Two protein toxins, Hvt (ω-atracotoxin from Hadronyche versuta) and onion leaf lectin, were translationally fused to evaluate the negative effects of fusion proteins on Phenacoccus solenopsis (mealybug), a phloem-feeding insect pest. Hvt was cloned both N-terminally (HL) and then C-terminally (LH) in the fusion protein constructs, which were expressed transiently in Nicotiana tabacum using a Potato Virus X (PVX) vector. The HL fusion protein was found to be more effective against P. solenopsis, with an 83% mortality rate, as compared to the LH protein, which caused 65% mortality. Hvt and lectin alone caused 42% and 45%, respectively, under the same conditions. Computational studies of both fusion proteins showed that the HL protein is more stable than the LH protein. Together, these results demonstrate that translational fusion of two insecticidal proteins improved the insecticidal activity relative to each protein individually and could be expressed in transgenic plants for effective control of sucking pests. |
| Databáze: | OpenAIRE |
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