Telmisartan prevents angiotensin II-induced endothelial dysfunction in rabbit aorta via activating HGF/Met system and PPARγ pathway
| Autor: | Yuan Wang, Bang-Ning Wang, Ze-Ping Hu, Nan Fang, Hai-Yan Qian, Xiao-Ling Fang |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Indoles Peroxisome proliferator-activated receptor Benzoates Polymerase Chain Reaction Piperazines Internal medicine medicine Animals Anilides Pharmacology (medical) Telmisartan Endothelial dysfunction Receptor Aorta Pharmacology chemistry.chemical_classification Sulfonamides Dose-Response Relationship Drug Chemistry Serine Endopeptidases Antagonist medicine.disease Angiotensin II PPAR gamma Endocrinology Benzimidazoles Hepatocyte growth factor Endothelium Vascular Rabbits Angiotensin II Type 1 Receptor Blockers Acetylcholine medicine.drug |
| Zdroj: | Fundamental & Clinical Pharmacology. 28:501-511 |
| ISSN: | 0767-3981 |
| Popis: | Telmisartan with partial activation of peroxisome proliferator-activated receptor γ (PPARγ) powerfully reduces blood pressure, improves endothelial function and lipid metabolism. Hepatocyte growth factor/mesenchymal-epithelial transition factor (HGF/Met) system in the local vasculature plays a pivotal role in maintaining normal endothelial function. This study is aimed to evaluate whether telmisartan directly prevents angiotensin II (Ang II)-induced endothelial dysfunction (ED) via activating HGF/Met system and/or PPARγ pathway. The isolated aortic rings of rabbits were incubated with Ang II (0.01-1 μM), telmisartan (0.1-10 μM), SU11274 (5 μM) as a specific Met inhibitor, GW9662 (10 μM) as a PPARγ antagonist alone or a combination for 6 h. Ang II obviously inhibited the mRNA and protein expression of HGF, Met and PPARγ, and the accumulative concentration-relaxation of the aortic rings to acetylcholine, among which the inhibitory effect of 1 μM Ang II was most significant. By contrast, telmisartan significantly increased the mRNA and protein expression of HGF, Met, and PPARγ, thus preventing Ang II-induced ED in a dose-dependent pattern. However, SU11274, GW9662 or a combination of both partially abolished the protective effects derived from telmisartan, with the effect of SU11274 exceeding that of GW9662. These results demonstrate that Ang II-induced ED in rabbit aortic rings in vitro can be prevented by telmisartan through selective PPARγ-modulating pathway. Moreover, this study indicates for the first time that activating HGF/Met system in the local vasculature is involved in the protective mechanism of telmisartan. |
| Databáze: | OpenAIRE |
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