Genetic characterization of Shigatoxigenic and enteropathogenic Escherichia coli O80:H2 from diarrhoeic and septicaemic calves and relatedness to human Shigatoxigenic E. coli O80:H2

Autor: Virginie Guérin, Keiji Nakamura, Damien Thiry, Tetsuya Hayashi, Jacques Mainil, Marc Saulmont, Florence Crombé, Audrey Habets, K De Rauw, Denis Pierard
Přispěvatelé: Supporting clinical sciences, Clinical Biology, Microbiology and Infection Control, Faculty of Physical Education and Physical Therapy
Rok vydání: 2020
Předmět:
Diarrhea
Serotype
Cattle Diseases
Biology
Enterohemorrhagic Escherichia coli/classification
phylogeny
Serogroup
medicine.disease_cause
Applied Microbiology and Biotechnology
Genome
Microbiology
Enteropathogenic Escherichia coli
03 medical and health sciences
Belgium
Sepsis
hemic and lymphatic diseases
Escherichia coli Infections/epidemiology
medicine
Animals
Humans
Potential source
Sepsis/epidemiology
Belgium/epidemiology
Gene
Escherichia coli
Escherichia coli Infections
Feces
030304 developmental biology
Whole genome sequencing
Cattle Diseases/epidemiology
0303 health sciences
030306 microbiology
Escherichia coli Proteins
Genome
Bacterial/genetics
General Medicine
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
Diarrhea/epidemiology
Enteropathogenic Escherichia coli/classification
Enterohemorrhagic Escherichia coli
bacteria
Cattle
Escherichia coli Proteins/genetics
Genome
Bacterial
Biotechnology
Zdroj: Journal of Applied Microbiology. 130:258-264
ISSN: 1365-2672
1364-5072
DOI: 10.1111/jam.14759
Popis: Aim The purpose of this work was to identify and genetically characterize enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) O80:H2 from diarrhoeic and septicaemic calves in Belgium and to comparing them with human EHEC after whole genome sequencing. Methods and results Ten EHEC and 21 EPEC O80 identified by PCR between 2009 and 2018 from faeces, intestinal content and a kidney of diarrhoeic or septicaemic calves were genome sequenced and compared to 19 human EHEC identified between 2008 and 2019. They all belonged to the O80:H2 serotype and ST301, harboured the eaeξ gene, and 23 of the 29 EHEC contained the stx2d gene. Phylogenetically, they were distributed in two major sub-lineages: one comprised a majority of bovine EPEC whereas the second one comprised a majority of stx2d bovine and human EHEC. Conclusions Not only EPEC but also EHEC O80:H2 are present in diarrhoeic and septicaemic calves in Belgium and are genetically related to human EHEC. Significance and impact of the study These findings support the need to assess cattle as potential source of contamination of humans by EHEC O80:H2 and to understand the evolution of bovine and human EHEC and EPEC O80:H2.
Databáze: OpenAIRE
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