The proteomic landscape of ovarian cancer cells in response to melatonin

Autor: Roberta Carvalho Cesário, Leticia Barbosa Gaiotte, Maira Smaniotto Cucielo, Henrique Spaulonci Silveira, Lucilene Delazari dos Santos, Debora Aparecida Pires de Campos Zuccari, Fábio Rodrigues Ferreira Seiva, Russel J. Reiter, Luiz Gustavo de Almeida Chuffa
Přispěvatelé: Universidade Estadual Paulista (UNESP), São José do Rio Preto, Universidade Estadual do Norte do Paraná – UENP, UT Health
Rok vydání: 2021
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1879-0631
Popis: Made available in DSpace on 2022-04-29T08:39:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-04-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Ovarian cancer (OC) is the most lethal gynecological malignancy with a highly negative prognosis. Melatonin is an indoleamine secreted by the pineal gland during darkness and has shown antitumor activity in both in vitro and in vivo experiments. Herein, we investigated the influence of melatonin on the proteome of human ovarian carcinoma cells (SKOV-3 cell line) using the Ultimate 3000 LC Liquid NanoChromatography equipment coupled to a Q-Exactive mass spectrometry. After 48 h of treatment, melatonin induced a significant cytotoxicity especially with the highest melatonin concentration. The proteomic profile revealed 639 proteins in the control group, and 98, 110, and 128 proteins were altered by melatonin at the doses of 0.8, 1.6, and 2.4 mM, respectively. Proteins associated with the immune system and tricarboxylic acid cycle were increased in the three melatonin-exposed groups of cells. Specifically, the dose of 2.4 mM led to a reduction in molecules associated with protein synthesis, especially those of the ribosomal protein family. We also identified 28 potential genes shared between normal ovarian tissue and OC in all experimental groups, and melatonin was predicted to alter genes encoding ribosomal proteins. Notably, the set of proteins changed by melatonin was linked to a better prognosis for OC patients. We conclude that melatonin significantly alters the proteome of SKOV-3 cells by changing proteins involved with the immune response and mitochondrial metabolism. The concentration of 2.4 mM of melatonin promoted the largest number of protein changes. The evidence suggests that melatonin may be an effective therapeutic strategy against OC. Department of Structural and Functional Biology Institute of Biosciences UNESP - Sao Paulo State University Biotechnology Institute (IBTEC) São Paulo State University (UNESP) Faculty of Medicine of São José do Rio Preto São José do Rio Preto Department of Biology Biological Science Center Universidade Estadual do Norte do Paraná – UENP, Luiz Meneghel Campus Departament of Cell Systems and Anatomy UT Health Graduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP) Department of Structural and Functional Biology Institute of Biosciences UNESP - Sao Paulo State University Biotechnology Institute (IBTEC) São Paulo State University (UNESP) Graduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP) FAPESP: 2019/00906-6 CNPq: 304108/2020-0 CAPES: 88887.482368/2020-00
Databáze: OpenAIRE
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