Human Immunodeficiency Virus Infection of Human-PBL-SCID Mice
| Autor: | Donald E. Mosier, Deborah H. Spector, Stephen M. Baird, Darcy B. Wilson, Stephen A. Spector, Richard J. Gulizia |
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| Rok vydání: | 1991 |
| Předmět: |
HIV Infections
Spleen In situ hybridization Biology Virus law.invention Pathogenesis Mice Peritoneal cavity Immune system law medicine Animals Humans Blood Transfusion Polymerase chain reaction Multidisciplinary Chimera Immunologic Deficiency Syndromes Virology Mice Mutant Strains Disease Models Animal medicine.anatomical_structure Lymphocyte Transfusion Immunology HIV-1 Lymph |
| Zdroj: | Science. 251:791-794 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.1990441 |
| Popis: | Severe combined immunodeficient (SCID) mice reconstituted with human peripheral blood leukocytes (hu-PBL-SCID mice) have inducible human immune function and may be useful as a small animal model for acquired immunodeficiency syndrome (AIDS) research. Hu-PBL-SCID mice infected with human immunodeficiency virus-1 (HIV-1) contained virus that was recoverable by culture from the peritoneal cavity, spleen, peripheral blood, and lymph nodes for up to 16 weeks after infection; viral sequences were also detected by in situ hybridization and by amplification with the polymerase chain reaction (PCR). Mice could be infected with multiple strains of HIV-1, including LAV-1/Bru, IIIB, MN, SF2, and SF13. HIV-1 infection affected the concentration of human immunoglobulin and the number of CD4+ T cells in the mice. These results support the use of the hu-PBL-SCID mouse for studies of the pathogenesis and treatment of AIDS. |
| Databáze: | OpenAIRE |
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