Inhibition of mouse mammary tumor virus-induced T cell responses in vivo by antibodies to an open reading frame protein
| Autor: | Leonardo Scarpellino, H R MacDonald, A N Shakhov, Werner Held, Hans Acha-Orbea |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
medicine.drug_class
T cell T-Lymphocytes viruses Immunology Molecular Sequence Data Receptors Antigen T-Cell Mice Inbred Strains chemical and pharmacologic phenomena Biology Monoclonal antibody Transfection Polymerase Chain Reaction Clonal deletion Cell Line Minor Lymphocyte Stimulatory Antigens Gene product Mice Open Reading Frames Amino Acid Sequence Animals Antibodies Monoclonal/immunology Baculoviridae/genetics Base Sequence DNA/genetics DNA/isolation & purification Immunity Cellular Mammary Tumor Virus Mouse/genetics Mammary Tumor Virus Mouse/immunology Mice Inbred BALB C/immunology Minor Lymphocyte Stimulatory Antigens/genetics Minor Lymphocyte Stimulatory Antigens/immunology Oligodeoxyribonucleotides Peptides/chemical synthesis Peptides/immunology Receptors Antigen T-Cell/genetics T-Lymphocytes/immunology medicine Immunology and Allergy Peptide sequence Mice Inbred BALB C Mouse mammary tumor virus T-cell receptor Antibodies Monoclonal DNA Articles biology.organism_classification Virology Molecular biology Open reading frame medicine.anatomical_structure Mammary Tumor Virus Mouse Peptides Baculoviridae |
| Zdroj: | Journal of Experimental Medicine, vol. 176, no. 6, pp. 1769-1772 The Journal of Experimental Medicine |
| Popis: | Minor lymphocyte stimulating (Mls) antigens specifically stimulate T cell responses that are restricted to particular T cell receptor (TCR) beta chain variable domains. The Mls phenotype is genetically controlled by an open reading frame (orf) located in the 3' long terminal repeat of mouse mammary tumor virus (MMTV); however, the mechanism of action of the orf gene product is unknown. Whereas predicted orf amino acid sequences show strong overall homology, the 20-30 COOH-terminal residues are strikingly polymorphic. This polymorphic region correlates with TCR V beta specificity. We have generated monoclonal antibodies to a synthetic peptide encompassing the 19 COOH-terminal amino acid residues of Mtv-7 orf, which encodes the Mls-1a determinant. We show here that these antibodies block Mls responses in vitro and can interfere specifically with thymic clonal deletion of Mls-1a reactive V beta 6+ T cells in neonatal mice. Furthermore, the antibodies can inhibit V beta 6+ T cell responses in vivo to an infectious MMTV that shares orf sequence homology and TCR specificity with Mtv-7. These results confirm the predicted extracellular localization of the orf COOH terminus and imply that the orf proteins of both endogenous and exogenous MMTV interact directly with TCR V beta. |
| Databáze: | OpenAIRE |
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