Implication of activation of intraperitoneal macrophages with bio-degradable microspheres
Autor: | K Mamba, T Okawa, T. Ishida, S Kaguba, Takashi Makita, H Ichimal, M. Kawata |
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Rok vydání: | 1989 |
Předmět: |
Male
Ratón Biology law.invention Peritoneal cavity Mice Peritoneum law polycyclic compounds medicine Animals Peritoneal Cavity Cells Cultured Mice Inbred ICR Chromatography Peritoneal fluid Petri dish Macrophages fungi Cell Biology biochemical phenomena metabolism and nutrition Carbohydrate Macrophage Activation Microspheres enzymes and coenzymes (carbohydrates) medicine.anatomical_structure Cell culture Immunology Microscopy Electron Scanning bacteria Electron microscope |
Zdroj: | Cell biology international reports. 13(6) |
ISSN: | 0309-1651 |
Popis: | To estimate the possibility that biological degradable starch microspheres (DMS) activate abdominal or intraperitoneal macrophages (IMP), two sizes of DMS (Spherex, Pharmacia, Sweden) were injected into the peritoneum of the ICR mice of 4 to 8 weeks of age. Three days after the injection, peritoneal fluid was collected and incubated for one hour at 37 degrees C under 5% CO2. The cells which adhered to the petri dish were IMP, to which DMS was added for 18 hrs. The cultured IMP were observed by scanning electron microscope (SEM) and the ratio of the active type to the total number of IMP was counted as an index of the effect of DMS to IMP. The activation effect of DMS on the incubated IMP was significant in the group which was cultured with 2 microns DMS after the 45 microns DMS injection. That indicated the possible DMS function as a potential IMP activating factor (MAF). |
Databáze: | OpenAIRE |
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