Directing cell therapy to anatomic target sites in vivo with magnetic resonance targeting
| Autor: | Jim M. Wild, N. Farrow, Claire E. Lewis, Melanie Paul, Fiona Wright, Russell Hughes, Joe Conner, Munitta Muthana, Craig Murdoch, Jon Dobson, Aneurin J. Kennerley, Mark F. Lythgoe, Ester Fagnano, Christopher Payne, Jay Richardson |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Gadolinium DTPA
Male Magnetic Resonance Spectroscopy Cell Transplantation Heterologous Cell- and Tissue-Based Therapy General Physics and Astronomy Contrast Media Metal Nanoparticles Ferric Compounds General Biochemistry Genetics and Molecular Biology Article Metastasis Cell therapy Capillary Permeability chemistry.chemical_compound Mice Random Allocation In vivo medicine Animals Humans Cells Cultured Multidisciplinary medicine.diagnostic_test Chemistry Macrophages Prostatic Neoplasms Magnetic resonance imaging General Chemistry Neoplasms Experimental medicine.disease equipment and supplies Oncolytic virus Transplantation Oncolytic Viruses medicine.anatomical_structure Immunology Cancer research human activities Iron oxide nanoparticles |
| Zdroj: | Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Cell-based therapy exploits modified human cells to treat diseases but its targeted application in specific tissues, particularly those lying deep in the body where direct injection is not possible, has been problematic. Here we use a magnetic resonance imaging (MRI) system to direct macrophages carrying an oncolytic virus, Seprehvir, into primary and metastatic tumour sites in mice. To achieve this, we magnetically label macrophages with super-paramagnetic iron oxide nanoparticles and apply pulsed magnetic field gradients in the direction of the tumour sites. Magnetic resonance targeting guides macrophages from the bloodstream into tumours, resulting in increased tumour macrophage infiltration and reduction in tumour burden and metastasis. Our study indicates that clinical MRI scanners can not only track the location of magnetically labelled cells but also have the potential to steer them into one or more target tissues. Cell therapy requires the targeting of cells to specific sites in the body. Here Muthana et al. use a standard MRI scanner to direct oncolytic macrophages, labelled with magnetic nanoparticles, to primary and metastatic tumour sites in mice, and demonstrate that this leads to reduced tumour growth. |
| Databáze: | OpenAIRE |
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