JAK2 ex13InDel drives oncogenic transformation and is associated with chronic eosinophilic leukemia and polycythemia vera
| Autor: | Nicholas C.P. Cross, Soo Jin Kim, Stefan N. Constantinescu, Dongqing Yan, Gerald J. Gleich, Jonathan Lambert, Emilie Leroy, Phillip M. Clair, Ami B. Patel, Anca Franzini, Michael Xiao, Jonathan M. Ahmann, Archana M. Agarwal, Lidvine Genet, Anthony D. Pomicter, Juanah Addada, Thomas O'Hare, Michael W. Deininger, Josef T. Prchal, Matthew Salmon |
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| Přispěvatelé: | UCL - SSS/DDUV/SIGN - Cell signalling, UCL - (SLuc) Service d'hématologie |
| Rok vydání: | 2019 |
| Předmět: |
Male
Signal transduction Biochemistry Myeloproliferative disease Mice Polycythemia vera INDEL Mutation hemic and lymphatic diseases Receptors Hypereosinophilic Syndrome Eosinophilia Chronic Polycythemia Vera Cells Cultured Aged 80 and over B-Lymphocytes Janus kinase 2 Leukemia biology Hematology Eosinophilic leukemia Cell Transformation Neoplastic Female medicine.symptom Cytokine receptor Adult Immunology Clonal Evolution medicine Animals Humans Amino Acid Sequence Myelofibrosis Cytokine Aged Chronic eosinophilic leukemia Essential thrombocythemia business.industry Jak2 gene v617f Phosphotransferases Cell Biology Oncogenes Janus Kinase 2 medicine.disease Mutation biology.protein Cancer research Interleukin-3 Janus kinase business |
| Zdroj: | Blood, Vol. 134, no. 26, p. 2388-2398 (2019) |
| ISSN: | 1528-0020 |
| Popis: | The V617F mutation in the JH2 domain of Janus kinase 2 (JAK2) is an oncogenic driver in several myeloproliferative neoplasms (MPNs), including essential thrombocythemia, myelofibrosis, and polycythemia vera (PV). Other mutations in JAK2 have been identified in MPNs, most notably exon 12 mutations in PV. Here, we describe a novel recurrent mutation characterized by a common 4-amino-acid deletion and variable 1-amino-acid insertion (Leu583-Ala586DelInsSer/Gln/Pro) within the JH2 domain of JAK2. All 4 affected patients had eosinophilia, and both patients with Leu583-Ala586DelInsSer fulfilled diagnostic criteria of both PV and chronic eosinophilic leukemia (CEL). Computational and functional studies revealed that Leu583-Ala586DelInsSer (herein referred to as JAK2ex13InDel) deregulates JAK2 through a mechanism similar to JAK2V617F, activates signal transducer and activator of transcription 5 and extracellular signal-regulated kinase, and transforms parental Ba/F3 cells to growth factor independence. In contrast to JAK2V617F, JAK2ex13InDel does not require an exogenous homodimeric type 1 cytokine receptor to transform Ba/F3 cells and is capable of activating β common chain family cytokine receptor (interleukin-3 receptor [IL-3R], IL-5R, and granulocyte-macrophage colony stimulating factor receptor) signaling in the absence of ligand, with the maximum effect observed for IL-5R, consistent with the clinical phenotype of eosinophilia. Recognizing this new PV/CEL-overlap MPN has significant clinical implications, as both PV and CEL patients are at high risk for thrombosis, and concomitant cytoreduction of red cells, neutrophils, and eosinophils may be required for prevention of thromboembolic events. Targeted next-generation sequencing for genes recurrently mutated in myeloid malignancies in patients with unexplained eosinophilia may reveal additional cases of Leu583-Ala586DelInsSer/Gln/Pro, allowing for complete characterization of this unique MPN. |
| Databáze: | OpenAIRE |
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