Separation of rosette-forming cells with the aid of an immunosorbent based on sephadex G-25 and G-75
| Autor: | I. V. Khazanova, B. B. Fuks, V. L. Yurin |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Hemolytic Plaque Technique
Cell Separation General Biochemistry Genetics and Molecular Biology Sepharose Rosette (botany) chemistry.chemical_compound Mice Animals Lymphocytes Bovine serum albumin Mice Inbred BALB C Chromatography biology Sodium periodate Serum Albumin Bovine General Medicine Immunosorbents chemistry Sephadex Covalent bond Antibody Formation biology.protein Adsorption Binding Sites Antibody Spleen |
| Zdroj: | Bulletin of experimental biology and medicine. 77(7) |
| ISSN: | 0007-4888 |
| Popis: | A method of separating rosette-forming cells on columns with an immunosorbent based on sephadex G-75 is described and has been tested. The protein antigen (bovine serum albumin-BSA) is attatched by covalent bonds to the surface of the Sephadex granules oxidized by sodium periodate. Cells with receptors on their surface were tested by the rosette method. Up to 88% of the rosette-forming cells were retained on a column packed with BSA-Sephadex granules. Nonspecific retention of the cells was relatively small, about 4%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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